美廉净凝胶对白假丝酵母菌致小鼠阴道炎的药效学研究

目的:以白假丝酵母菌感染雌激素化的小鼠形成阴道炎模型,并探讨美廉净凝胶对白假丝酵母菌性阴道炎的药效作用.方法:建立白假丝酵母菌感染小鼠阴道炎模型,设置模型组,双唑泰组,保妇康凝胶组,美廉净凝胶高、中、低剂量组,阴道给药每天2次,连续14 d.测定小鼠血清中白细胞介素-6(interleukin-6,IL-6)、NO(N...

Pharmacodynamic study on Meilianjing gel for the therapy of vaginitis in mice caused by Candida albicans

OBJECTIVE To evaluate the curative effects of Meilianjing temperature-sensitive gel on a mouse model of vaginitis induced by C. albicans.METHODS The mouse model of vaginitis was induced by C. albicans, and then divided into model group, Shuangzuotai group, Baofukang gel group, and low, medium and high dose of Meilianjing gel groups. Except those in the model group, the mice in the other groups were treated with corresponding drug twice a day for 14 consecutive days. After treatment, the expression levels of interleukin-6 (IL-6) and NO in serum of mice were measured. Furthermore, the vaginal pathogen conversion rate and vaginal tissue inflammation degree were analyzed to determine the curative effects of Meilianjing gel.RESULTS Compared with the model group, Meilianjing gel could inhibit the growth and reduce the number of colonies of C. albicans in mouse vagina. The edema and inflammation of mouse vagina were also attenuated after treatment, while Meilianjing gel showed little effect of secretion inhibition. Meanwhile, Meilianjing gel had little effect on the liver index and vaginal index, but it could increase the spleen and thymus index. The contents of IL-6 and NO in serum in every drug treatment group were lower than those in the model group (P＜0.01). HE staining showed that Meilianjing gel could reduce vaginitis, and PAS staining showed that it could reduce the infection and glycogen reduction of C. albicans.CONCLUSION Meilianjing gel can attenuate the vaginitis by inhibiting the proliferation of C. albicans and reducing the expression of IL-6 and NO in serum of mice.