脓毒症肠道功能障碍小鼠肠道组织多肽组学分析

目的 观察脓毒症肠道功能障碍小鼠肠组织多肽谱差异表达，探索脓毒症肠道功能障碍机制。方法 采用盲肠结扎穿孔法建立脓毒症肠道功能障碍小鼠模型，分假手术组(n=3)和脓毒症组(n=3)，取回肠组织，运用液相色谱串联质谱法检测肠组织多肽谱，差异表达内源性多肽进行生物信息学分析，推断其在脓毒症肠功能障碍中的作用。结果 共鉴定到458条多肽序列，涉及129个前体蛋白，得出差异上调的多肽有101个，下调的多肽有9个，差异内源性多肽可能通过调控代谢途径、抗原处理和呈递、氧化磷酸化、MAPK信号通路、细胞凋亡等参与脓毒症肠道功能障碍的发生发展，并预测出脓毒症肠道功能障碍相关的15条内源性多肽。结论 发现了15条差异表达内源性多肽，可能是参与脓毒症肠道功能障碍的关键生物活性多肽，为脓毒症肠道功能障碍的防治提供了新的方向

Peptidomics analysis of intestinal tissues in mice with sepsis-induced intestinal dysfunction

Objective To observe differential peptidomics of intestinal tissues in mice with sepsis-induced intestinal dysfunction，and to investigate the mechanism of sepsis-induced intestinal dysfunction. Methods The mice model of sepsis-induced intestinal dysfunction was established by cecal ligation and perforation and divided into sham operation group (3 mice) and sepsis group (3 mice).The peptidomic profiling of intestinal tissue was detected by liquid chromatography-tandem mass spectrometry，and the differentially expressed endogenous peptides were subjected to bioinformatics analysis to assess their possible roles in sepsis-induced intestinal dysfunction. Results A total of 458 peptides and 129 precursor proteins were identified，and 110 of them were differentially expressed，including 101 upregulated peptides and 9 downregulated peptides. Differential endogenous peptides may be involved in the occurrence and development of sepsis-induced intestinal dysfunction through regulation of metabolic pathways，antigen processing and presentation， oxidative phosphorylation，MAPK signaling pathway，cell apoptosis， etc. And 15 endogenous peptides related to sepsis-induced intestinal dysfunction were predicted. Conclusion We found 15 differentially expressed endogenous peptides，which may be the key bioactive peptides involved in sepsis-induced intestinal dysfunction. This study provides a new direction for the prevention and treatment of intestinal dysfunction in sepsis.