薯蓣皂苷元在口腔鳞癌中的抗癌作用研究

目的 探究薯蓣皂苷元对口腔鳞癌 HSC4 细胞生物学行为的影响。 方法 将 HSC4 细胞分为薯蓣 皂苷元 0、 0. 5、 1、 2 μmol / L 4 组, CCK-8、 5-溴-2′-脱氧尿苷 (BrdU) 染色、 克隆形成实验、 流式细胞术、 划痕实验和 Transwell 实验分别检测细胞增殖、 凋亡、 迁移和侵袭, 免疫印迹检测 Bcl-2、 Bax 和 cleaced caspase-3 蛋白表达水平; JC-1 检测线粒体膜电位; H2DCFDA 检测活性氧 (ROS) 产生; 试剂盒检测谷胱 甘肽 (GSH) 和丙二醛 (MDA) 水平。 结果 与薯蓣皂苷元 0 μmol / L 组比较, 薯蓣皂苷元 1 μmol / L 组和 2 μmol / L 组细胞活力、 集落形成能力、 BrdU 阳性细胞率、 迁移和侵袭能力、 线粒体膜电位降低, 细胞凋亡 率升高, Bax 和 cleaced caspase-3 蛋白表达上调, Bcl-2 蛋白表达下调, ROS 和 MDA 水平升高, GSH 水平降 低。 结论 薯蓣皂苷元可抑制 HSC4 细胞的增殖和运动能力, 通过线粒体途径和 ROS 的产生诱导细胞凋亡。

Anticancer Effect of Diosgeninon Oral Squamous Cell Carcinoma

Objective To investigate the effect of diosgenin on the oral squamous cell carcinoma HSC4 cells. Methods HSC4 cells were divided into four groups: diosgenin 0, 0. 5, 1 and 2 μmol / L. Cell proliferation, apoptosis, migration and invasion were assayed by CCK-8, 5-bromo2′-deoxyuridine (BrdU) staining, colony formation experiment, flow cytometry, wound healing assay and transwell assay. The protein expression levels of Bcl-2, Bax and cleaced caspase-3 were detected by Western blotting. JC-1 was employed to measure the mitochondrial membrane potential. H2DCFDA was used to detect the production of reactive oxygen species (ROS). GSH and MDA levels were detected by the kits. Results The cell viability, colony formation ability, BrdU positive cell rate, cell migration and invasion ability, mitochondrial membrane potential, GSH level, and the expression level of Bcl-2 were decreased in the 1 μmol / L and 2 μmol / L diosgenin groups when compared with the 0 μmol / L diosgenin group. The apoptosis rate, the expression levels of Bax and cleaced caspase-3, and the ROS and MDA levels were increased in the 1 μmol / L and 2 μmol / L diosgenin groups when compared with the μmol / L diosgenin group. Conclusion Diosgenin can inhibit the proliferation and motility of HSC4 cells, and induce apoptosis through mitochondrial pathway and ROS production.