功能性便秘患儿的肠道菌群分析及治疗干预

目的 分析功能性便秘（FC）患儿肠道菌群含量的变化及非药物基础治疗的临床疗效。方法 选取2019年9月至2021年10月在昆明医科大学附属儿童医院消化内科门诊诊断为FC的患儿30例作为研究对象，选取同期来该院儿童保健科体检的30例健康儿童作为正常对照组。收集FC患儿经饮食结构调整、定时排便训练、适量运动等基础治疗前、治疗后和正常对照组的粪便标本。利用昆明合和医学检验所的MiNi8 plus荧光定量PCR仪测定各组儿童粪便中的肠道菌群含量。同时观察FC患儿经基础治疗后便秘症状的缓解情况。结果 FC患儿双歧杆菌属、乳酸杆菌属含量显著低于正常对照组，差异有统计学意义（P <0.01）。FC患儿经基础治疗后乳酸杆菌属含量较治疗前组明显增多，差异有统计学意义（P <0.01）。FC患儿经基础治疗后便秘症状评分均较治疗前组降低，差异有统计学意义（P <0.01）。结论 FC患儿会出现肠道菌群紊乱，经饮食结构调整、生活方式改善等基础治疗后肠道菌群紊乱和便秘症状得以改善。

Analysis of Intestinal Flora and Therapeutic Intervention in Children with Functional Constipation

  Objective  To analyze the changes of intestinal flora in children with functional constipation （FC） and the clinical significance of non-pharmacological basic treatment.   Methods  A total of 30 children with FC were selected from the outpatient department of the Children's Hospital affiliated to Kunming Medical University between September 2019-October 2021, and 30 health children were selected as control group. The children with FC were given basic treatment that including dietary structure adjustment, regular defecation training and appropriate exercise.The fecal samples were collected respectively in the pre-treatment group and post-treatment group from the children with FC and from the healthy children. The contents of intestinal flora in the stools of children in each group were measured by the MiNi8 plus fluorescence quantitative PCR instrument from the Kunming Hehe Medical Laboratory. In addition, the relief of constipation symptoms after basic treatment in children with FC was observed.   Results  The content of lactobacillus and bifidobacterium in children with FC was significantly lower than that in control group , and the difference was statistically significant （P < 0.01）. The content of lactobacillus in the post-treatment group was significantly higher than that in the pre-treatment group, and the difference was statistically significant （P < 0.01）. The scores of constipation symptoms in children with FC after basic treatment were lower than those in pre-treatment group, and the difference was statistically significant （P < 0.01）.   Conclusions  The children with FC will have intestinal flora disorder. After basic treatment such as dietary structure adjustment and lifestyle improvement, the intestinal flora disorder and constipation symptoms can be improved.