| 抑癌基因p27对增生性瘢痕成纤维细胞增殖及DNA合成的影响 |
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| 引用本文： | 董茂龙,陈璧. 抑癌基因p27对增生性瘢痕成纤维细胞增殖及DNA合成的影响[J]. 医学争鸣, 2002, 23(18): 1645-1647 |
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| 作者姓名： | 董茂龙 陈璧 |
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| 作者单位： | 第四军医大学西京医院烧伤外科,陕西,西安,710033;第四军医大学西京医院烧伤外科,陕西,西安,710033 |
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| 摘 要： | 目的：探讨抑癌基因p27对增生性瘢痕的作用。方法：取体外培养的人增生性瘢痕成纤维细胞（HTsFb），将构建的pcDNA3-p27真核表达质粒体外转染HTsFb，经G418筛选获得稳定性表达的细胞克隆，用MTT和^3H-TdR掺入法观察抑癌基因p27对HTsFb增殖及DNA合成的影响。结果：抑癌基因p27对HTsFb增殖及DNA合成均有显著抑制作用。结论：抑癌基因p27可能通过抑制成纤维细胞增殖及DNA合成抑制瘢痕增生。
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| 关 键 词： | p27 MTT实验 DNA/生物合成 3H-TdR掺入 增生 瘢痕 肥大性 成纤维细胞 |
| 文章编号： | 1000-2790(2002)18-1645-03 |
| 修稿时间： | 2002-03-15 |
Effects of tumor suppression gene p27 on proliferation and DNA synthesis of hypertrophic scar fibroblasts |
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| DONG Mao-Long,CHEN Bi. Effects of tumor suppression gene p27 on proliferation and DNA synthesis of hypertrophic scar fibroblasts[J]. Negative, 2002, 23(18): 1645-1647 |
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| Authors: | DONG Mao-Long CHEN Bi |
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| Affiliation: | DONG Mao-Long,CHEN Bi Department of Burn Surgery,Xijing Hospital,Fourth Military Medical University,Xi'an 710033,China |
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| Abstract: | AIM To investigate the effect of tumor suppression gene p27 on the forming of hypertrophic scars. METHODS The eukaryotic expression plasmid pcDNA3-p27 for tumor suppression gene p27 was transduced into cultured human hypertrophic scar fibroblasts in vitro. MTT and 3H-TdR incorporation were applied to measure the effect of p27 on the proliferation and DNA synthesis of hypertrophic scar fibroblasts. RESULTS Tumor suppression gene p27 inhibited the proliferation and DNA synthesis of hypertrophic scar fibroblasts. CONCLUSION Tumor suppression gene p27 may inhibit scar forming by inhibiting the proliferation and DNA synthesis of fibroblasts. |
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| Keywords: | p27 MTT test DNA/biosynthesis 3H-TdR incorporation hyperplasia cicatrix hypertrophic fibroblasts |
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