| 套式PCR扩增特定SSU rRNA基因片段检测四川疟原虫感染的研究 |
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| 引用本文： | 姜素华,刘佩娜,朱声华,刘庆,陈建平.套式PCR扩增特定SSU rRNA基因片段检测四川疟原虫感染的研究[J].寄生虫病与感染性疾病,2001,9(1):4-7. |
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| 作者姓名： | 姜素华 刘佩娜 朱声华 刘庆 陈建平 |
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| 作者单位： | 1. 华西医科大学寄生虫学教研室,
2. Infectious Disease Section Department of Internal Medicine ,Yale University School of Medicine , |
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| 基金项目： | 日本丰田基金(96B3-011) |
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| 摘 要： | 目的用套式PCR系统诊断、鉴别人体疟原虫感染。方法以疟原虫小亚单位核糖体核糖核酸（SSUrRNA）基因为靶基因，选用1对疟原虫属特异性引物和4对种特异性引物，建立套式PCR扩增系统并用于四川省疟疾病人血样的检测。结果从间日疟原虫、恶性疟原虫和三日疟原虫感染血样中分别扩增出104bp、102bp和115bp预期大小的特定扩增带。61份血样检测结果与镜检的间日疟阳性符合率为100％。并查出镜检漏诊的2例P.v.和P.m.的混合感染及1例P.v.、P.f.和P.m.的混合感染病例。结论本系统特异、灵敏、稳定、简便，可在诊断疟疾的同时判定混合感染，故对疟疾的诊断、大规模流行病学研究及疫情监控有实际应用价值。
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| 关 键 词： | 间日疟原虫 恶性疟原虫 三日疟原虫 卵形疟原虫 套式聚合酶链反应 小亚单位核糖体核糖核酸基因 |
| 文章编号： | 1005-2534(2001)01-0004-04 |
| 修稿时间： | 2000年11月10 |
DETECTION OF HUMAN MALARIA PARASITES BASED ON AMPLIFYING SPECIFIC SSU rRNA GENE FRAGMENTBY NESTED PCR IN SICHUAN, CHINA |
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| JIANG Su-hua,LIU Pei-na,ZHU Sheng-hua,LIU Qing,CHEN Jian-ping.DETECTION OF HUMAN MALARIA PARASITES BASED ON AMPLIFYING SPECIFIC SSU rRNA GENE FRAGMENTBY NESTED PCR IN SICHUAN, CHINA[J].Parastoses and Infectious Diseases,2001,9(1):4-7. |
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| Authors: | JIANG Su-hua LIU Pei-na ZHU Sheng-hua LIU Qing CHEN Jian-ping |
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| Affiliation: | JIANG Su-hua1,LIU Pei-na1,ZHU Sheng-hua1,LIU Qing2,CHEN Jian-ping1 |
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| Abstract: | Aim To detect and identify human malaria parasites by nested PCR. Methods A pair of genus primer and four pairs of species-specific primers are chosen to establish nested PCR system and the targets specific sequence in the SSU rRNA gene are used for detecting blood samples obtained from malaria patients in Sichuan. Results 104bp,102bp and 115bp DNA expected fragments were amplified from the blood samples of 61 patients with P.vivax ,P.falciparum and P.malariae infections respectively. The positive concordance rate between nested PCR and microscopic examination was 100% in detecting P.vivax. In addition , 3 cases of mixed Plasmodium infections were identified by nested PCR. Conclusion The system is specific, sensitive and stable in detecting mixed malatia infections. It would provide a practical tool in diagnosis and epidemiological survey of malaria. |
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| Keywords: | Plasmodium vivax Plasmodium falciparum Plasmodium malariae Plasmodium ovale nested polymerase chain reaction small subunit ribosomal RNA gene |
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