GC法同时测定芫花中棕榈酸与亚油酸的含量

目的建立同时测定芫花药材中棕榈酸和亚油酸含量的气相色谱分析方法。方法采用毛细管气相色谱法,甲酯化后测定芫花中棕榈酸与亚油酸的含量。采用DB-17石英毛细管柱(30 m×0.25 mm,0.25μm);氢火焰离子化检测器(FID);程序升温:起始温度180℃,以5℃.min-1升至230℃;进样口温度:250℃;检测器温度:270℃;载气为氮气,流速:1.2 mL.min-1;分流比为20∶1。结果棕榈酸甲酯和亚油酸甲酯的质量浓度分别在0.079~1.578 g.L-1(r=0.999 6)和0.029~0.590 g.L-1(r=0.999 7)内与峰面积呈良好的线性关系;平均回收率(n=9)分别为98.1%(RSD=2.7%)和99.7%(RSD=2.8%)。结论本方法简便、快速、准确,为芫花的质量控制方法提供依据。

Simultaneous determination of the contents of palmitic acid and linoleic acid in Genkwa Flos by GC

Objective To establish a method for simultaneous determination of palmitic acid and linoleic acid in the Genkwa Flos.Methods The sample was determined with DB-17 capillary column(30 m×0.25 mm,0.25 μm)by GC after esterification.The detector was FID;temperature programs was 180 ℃ programmed to 230 ℃ at 5 ℃·min-1;the inlet temperature was 250 ℃;the detector temperature was 270 ℃.Nitrogen was used as carrier gas(1.2 mL·min-1).Split injection was conducted with split ratio of 20∶1.Results The linear ranges of methyl palmitate and methyl linoleate were 0.042-0.844 g·L-1(r=0.999 6)and 0.029-0.590 g·L-1(r=0.999 7),respectively;the average recoveries(n=9)were 98.1% with RSD of 2.7% and 99.7% with RSD of 2.9%,respectively.Conclusions The method is simple,quick and accurate,which is helpful for the quality control of Genkwa Flos.