不同种属动物胃窦粘膜中生长抑素免疫反应细胞的定量研究

目的　了解生长抑素免疫反应细胞 (SS -IR细胞 )在不同种属动物胃窦粘膜中的形态分布。方法　用免疫组织化学方法结合图像分析技术研究家兔、大鼠和小鼠胃窦粘膜中SS -IR细胞的数目和细胞的灰度。结果3种动物胃窦粘膜中SS -IR细胞主要分布于幽门腺的中、下 1/3处 ,在大鼠偶尔可见SS -IR细胞位于粘膜上皮中。SS -IR细胞多散在分布于幽门腺细胞之间 ,偶见几个SS -IR细胞聚在一起。在家兔胃窦粘膜中SS -IR细胞多为圆形 ,较少见到有长的胞质突起。在大鼠、小鼠胃窦粘膜中SS -IR细胞大小不等 ,形态多样。多见细胞发出细长的胞质突起 ,伸向邻近细胞 ;偶见有的细胞顶部可达腺腔。大鼠胃窦粘膜SS -IR细胞的数目比家兔、小鼠胃窦粘膜多 ,细胞平均灰度值小 (P <0 .0 1)。结论　胃窦粘膜中SS -IR细胞的形态及数量在不同种属动物间存在差异

A quantitative study on the somatostatin immunoreactive cells in the antral gastric mucosa of different species of animal

Objective To explore the differences in morphology and distribution of somatostatin immunoreactive cells (SS-IR cells) in the antral gastric mucosa of different species of animal. Methods The number and grey level of the positive SS-IR cells were determined by using the immunohistochemical method combined with image analysis technique. Results The SS-IR cells were predominantly located in the middle lower zone of the pyloric gland in all species, occasionally found in the gastric mucosal epithelium in rat. Most of the SS-IR cells were scattered among the other glandular cells, and a few of them were aggregated. The shape of the SS-IR cells in the gastric antrum of rabbit were mainly round in shape, with few protrusions on the surface. In rats and mice, the SS-IR cells in the gastric antrum were diverse in number and shape, with long thin protrusions projecting into the adjacent tissue, and some of the cells were lining the glandular lumen. The number and grey level of the SS-IR cells in gastric antrum were higher in rats than in rabbits and mice (P<0.01). Conclusion The results indicate that there are differences in morphology and number of SS-IR cells in the gastric antrum between different species of animal.