P2X4 Receptor Regulates Alcohol-Induced Responses in Microglia |
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| Authors: | Larisa Gofman Jonathan M Cenna Raghava Potula |
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| Institution: | 1. Department of Pathology and Laboratory Medicine, Temple University School of Medicine, 3500 N. Broad Street, MERB 845A, Philadelphia, PA, 19140, USA
2. Center for Substance Abuse Research, Temple University School of Medicine, Philadelphia, PA, USA
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| Abstract: | Mounting evidence indicates that alcohol-induced neuropathology may result from multicellular responses in which microglia cells play a prominent role. Purinergic receptor signaling plays a key role in regulating microglial function and, more importantly, mediates alcohol-induced effects. Our findings demonstrate that alcohol increases expression of P2X4 receptor (P2X4R), which alters the function of microglia, including calcium mobilization, migration and phagocytosis. Our results show a significant up-regulation of P2X4 gene expression as analyzed by real-time qPCR (***p?p?CX3CL1) by 75 % following 48 h of treatment compared to control (***p?CX3CL1-dependent migration was confirmed to be P2X4 receptor-dependent using the antagonist 5-BDBD, which reversed the effects as compared to alcohol alone (***p?p?p?
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