淫羊藿水提液防治去势大鼠骨质疏松症的实验研究

目的 探讨淫羊藿水提液防治去势大鼠骨质疏松症的疗效。方法 采用3月龄SD雌性大鼠30只，随机分为正常对照组(1组)、去势组(2组)和实验组(3组)。2组及3组均切除双侧卵巢，3组给予淫羊藿水提液灌胃处理；1组给予假手术处理。分别于手术后4周、12周，随机处死三组大鼠中各5只，取第五腰椎，脱钙后包埋切片。Van Gieson染色，图像分析骨小梁结构；HE染色，计数多核巨噬细胞／破骨样细胞。结果去势组与正常对照组比较，4周时出现骨小梁厚度下降，12周时骨小梁面积百分比下降明显，骨小梁分离度增加。实验组在骨小梁计量上与对照组相似，与去势组比较具有显差异。去势组多核巨噬细胞／破骨样细胞计数多于正常对照组及实验组，有显意义。结论 淫羊藿水提液通过抑制破骨细胞的生成，能有效地预防大鼠卵巢切除后所致骨质疏松症的发生。

Epimedium prevent ovariectomy-induced osteoporosis in rats

Objective To study the therapeutic results of Epimedium preventing o-variectomy-induced osteoporosis in rats. Methods 30 female SD rats at 3 months of age were randomly divided into control group (1 groups), tendency group(2 groups) and experiment group(3 groups). 2 group and 3 group all been amputated ovary,3 group were given Epimedium. 1 group were given to use skill handling. After 4 weeks and 12 weeks, random execute 5 rats in 3 group. Take the lumbar 5th vetebra and make paraffin section,stain with HE and Van Gieson,analyse bone little beam structure with image analyst,and count multi-nuclei/osteoclast like-cells. Results Tendency group compare with control group, Tb. Th and Tr. Ar have obvious decreasing while Tb. Sp increasing. Trabecular bone in Epimedium group measure is similar to compare group. The number of multi-nuclei/osteoclast like-cells in tendency group is obviously more than 1 group and 3 group. Conclusion Epimedium can prevent efficiently the ovariectomy-induced osteoporosis.