丛枝菌根真菌对半夏产量及化学成分的影响

目的:研究丛枝菌根真菌(AM)对半夏叶形指数、繁殖系数、产量及化学成分等的影响.方法:半夏生长期间常规测量叶长、叶宽、株高及叶柄直径(茎粗)直至倒苗,计算叶形指数;收获块茎后,测量并计算块茎产量;采用高效液相色谱法测量收获块茎和赫章县栽培半夏块茎中鸟苷含量;采用紫外-可见光分光光度计法测量收获块茎和赫章县栽培半夏块茎中生物碱含量.结果与结论:接种AM真菌可显著提高半夏块茎产量,提高繁殖系数;接种AM真菌提高半夏叶形指数,使叶片趋向竹叶型,推迟半夏倒苗时间;摩西球囊霉+根内球囊霉第1批处理(gm+gi 1)对提高半夏块茎鲜重、干重及繁殖系数的效果最为明显,说明多菌协同、接种时间越长对提高半夏块茎产量和繁殖系数效果越为明显.接种AM真菌可显著提高半夏块茎鸟苷、生物碱含量,且接种菌剂的处理生物碱及鸟苷含量大多高于赫章县栽培半夏;摩西球囊霉+根内球囊霉第2批处理(gm+gi 2)对增加块茎鸟苷、生物碱含量的效果最为明显,说明多菌协同,且在半夏生长中期进行接种效果最好.

Study on influence of arbuscular mycorrhizal fungi Pinellia ternatayield and chemical composition

Objective : To study the influence of arbuscular mycorrhizal fungi to Pinellia ternata leaf index, propagation coefficient, tuber yield and chemical composition. Method : The Pinellia ternata leaf length, width, plant height and leafstalk diameter were measured during growing time till P. ternata loded. The leaf index was calculated and the tuber yield were measured after harvest. The contents of guanosine was determined by HPLC method, and the alkaloid contents were determined by spectrophotometric method. Result and Conclusion : Inoculated AM fungi could increase P. ternata propagation coefficient and the yield of tuber. Inoculated AM fungi could increase P. ternata leaf index, made leaf tends to be more like bamboo leaf type. Inoculated AM fungi could delayed P. ternata lodging time; G. mosseae+ G. intraradices treatment (early inolulation time) proved better influence on increasing fresh weight and dry weight of P. ternata tuber and the propagation coefficient. Inoculated AM fungi could increase guanosine and alkaloid contents of Pinellia ternata tuber, and the contents of guanosine and alkaloid in inoculated treatments were higher than those in cultivated P. ternata from Hezhang; G. mosseae+ G. intraradices treatment (later inolulation time) proved good effect on increasing the contents of guanosine and alkaloid.