CML病人外周血T细胞CD3基因表达模式变化

在我们前期研究发现慢性髓系白血病（CML）病人中T细胞受体信号传导分子CD3ζ基因表达下调的基础上，本研究进一步分析病个外周血中全部4种CD3γδεζ基因的表达模式特点，以深入了解病人的T细胞免疫中信号转导的变化。利用SYBR Green I实时荧光定量RT—PCR检测17例初诊慢性期CML病人外周血单个核细胞中CD3γ、δ、ε和（链的表达水平，以132微球蛋白基因（132MG）作为内参照，采用相对定量公式：2^-△Ct×100％，计算CD3分子各亚单位的相对mRNA表达量，以17例健康成人外周血作为对照。结果表明：CML中CD3γ、δ、ε基因表达水平分别为2．344％，0．515％和3．516％（中位数），与健康对照组比较（3．093％，0．853％和2．302％）并没有显著性差异（P=0．072，P=0．190，P=0．615），而CML中CD3ζ链基因的表达水平（0．395％）则明显低于健康对照组（1．538％）（P〈0．001）。在CML中4种CD3分子表达水平模式依次为CD3ε〉CD3γ〉CD3δ〉CD3ζ，而对照组中则为CD3γ〉CD3ε〉CD3δ〉CD36ζ结论：本研究首先提供了CML病人中CD3各基因表达模式情况，显示以CD3ζ为主的TCR信号分子的表达缺陷，4种CD3链分子的表达水平模式也发生变化。

Change of Expression Pattern of CD3 Genes in Peripheral Blood T-Cells from CML Patients

Our previous finding showed that down-regulation of CD3ζ gene was detected in patients with chronic my eloid leukemia（CML）. In order to further elucidate the feature of T cell immune status in the signal transduction in CML patients, the expression patterns of all 4 CD3 genes were characterized in peripheral blood of patients, the expression levels of CD3γ, δ, ε and ξ chain genes were detected by real time qPCR with SYBR Green I staining in peripheral blood mononuclear cells （PBMNCs） from 17 cases of de novo CML patients in chronic phase and 17 cases of healthy individuals, the β2-microglobulin gene was used as an internal reference, and the mRNA expression level of each CD3 gene was evaluated by the 2 - △Cr × 100% method. The results showed that the median expression levels of CD3γ, δ and ε genes （2. 344% ,0. 515% and 3. 516% ） in CML patients were not significantly different from healthy individuals （p =0. 072, p =0. 190,p = 0. 615, respectively）, while the expression level of CD3ζ gene in PBMNCs from CML patients （0. 395% ） was lower than that from healthy individuals （ 1. 538% ） （p 〈 0. 001 ）. The expression patterns of 4 CD3 genes in proper order were CD3ε 〉 CD3γ 〉 CD3δ 〉 CD3ζ in CML group, in contrast, the expression patterns were pres- ented as CD3γ〉 CD3ε 〉 CD3δ 〉 CD3ζ in healthy group. It is concluded that the present study characterized the expression pattern of CD3γ, δ, ε and ζchain genes in CML patients, lower expression of CD3ζ is the feature of TCR signal transduction immunodeficiency and the expression patterns of 4 CD3 genes are changed in CML patients.