| TLR3激动剂对B细胞功能的影响 |
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| 引用本文： | 钱莉,傅奕,潘兴元,田芳,龚卫娟,季明春.TLR3激动剂对B细胞功能的影响[J].免疫学杂志,2011(10):833-836. |
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| 作者姓名： | 钱莉 傅奕 潘兴元 田芳 龚卫娟 季明春 |
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| 作者单位： | 扬州大学医学院病原生物学与免疫学教研室;扬州大学医学院生物化学教研室; |
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| 基金项目： | 国家自然科学基金(81001308,30800182); 江苏省自然科学基金(BK2010315); 扬州大学高层次人才科研启动资金(0474910015048) |
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| 摘 要： | 目的观察TLR3激动剂Poly I:C对B细胞功能的影响,包括细胞增殖、共刺激分子表达、细胞因子和抗体的分泌情况。方法利用免疫磁珠法分选小鼠脾脏CD19+B细胞,RT-PCR法证实其表达TLR3。体外用Poly I:C刺激B细胞一定时间后,CFSE分裂法检测B细胞增殖,流式细胞术(flow cytometry,FCM)检测B细胞表面共刺激分子表达,CBA(cytometric bead ar-ray)法或ELISA法检测培养上清中细胞因子含量,CBA法检测B细胞分泌Ig的亚型。结果 Poly I:C可以促进B细胞增殖,上调B细胞表面CD40、CD80、CD86和MHC II类分子的表达,促进IL-6和TNF-α的高分泌,诱导IgG1κ抗体的产生。结论 Poly I:C可以通过促进增殖、细胞因子分泌,诱导抗体产生和上调共刺激分子表达等多方面调节B细胞功能。
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| 关 键 词： | TLR3 B细胞 功能 |
Effects of TLR3 agonist on B cell functions |
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| QIAN Li,FU Yi,PAN Xinyuan,TIAN Fang,GONG Weijuan,JI Mingchun.Effects of TLR3 agonist on B cell functions[J].Immunological Journal,2011(10):833-836. |
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| Authors: | QIAN Li FU Yi PAN Xinyuan TIAN Fang GONG Weijuan JI Mingchun |
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| Institution: | QIAN Li,FU Yi,PAN Xinyuan,TIAN Fang,GONG Weijuan,JI MingchunDepartment of Etiology and Immunology,Medical School of Yangzhou University,Yangzhou 225001,Chin |
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| Abstract: | To explore the immunomodulatory effects of Poly I:C(TLR3 agonist) on B cells,freshly purified splenic B cells or CFSE-labeled B cells were cultured in presence or absence of PolyI:C.After 24 h,culture supernatants were collected for screening of cytokine secretion by ELISA or cytometric bead array(CBA) technology.At day 6 and 9,the dilution of CFSE was assayed by flow cytometry(FCM);at day 2 and 5,cells were analyzed for costimulatory molecules expression by FCM.At day 5,culture supernatants were collected ... |
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| Keywords: | TLR3 B cells Function |
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