前列腺癌组织中miRNAs的表达及意义

目的研究前列腺癌组织中6种miRNAs的表达及其意义,并探讨它们在前列腺癌诊断中的应用价值。方法采用核酸分子原位杂交(in situ hybridizationI,SH)技术,结合组织微阵列平台分别检测38例良性前列腺增生(benign prostate hyperpla-sia,BPH)和52例前列腺癌(prostatic cancer,PCa)中6种miRNAs的表达情况。结果 6种miRNAs在PCa中的表达率与BPH比较差异均有统计学意义(P<0.05)。6种miRNAs的表达均与PCa的Gleason评分相关(P<0.05);与患者的年龄及血清PSA水平均无明显相关性(P>0.05)。miR-15b、miR-182和miR-96的表达与PCa的临床分期相关(P<0.05);miR-96的表达与肿瘤累及前列腺叶数相关(P<0.05)。结论 miRNAs与PCa的发生、发展以及生物学行为有关,并可能作为PCa诊断及判断预后的生物标记物。

Expression of miRNAs in prostate cancer and its significance

Purpose To investigate the expression of 6 individual miRNAs and their significance in prostate cancer,and to discuss their use for the diagnosis of prostate cancer.Methods In situ hybridization staining combined with tissue microarray technology for 6 miRNAs was performed in 38 samples of benign prostate hyperplasia and 52 samples of prostate cancer.Results The expression of all 6 miRNAs showed a significant difference between the prostate cancer and benign prostatic hyperplasia tissues(P<0.05).The positive rate of these 6 miRNAs was significantly related to the Gleason grade of prostate cancer(P<0.05),but not with age and serous PSA level of the patient(P>0.05).On the otherhand,the expression of miR-15b,miR-182 and miR-96 correlated with tumor clinical stage(P<0.05),The expression of miR-96 correlated with the extension of tumor invasion in prostate(P<0.05).Conclusions The miRNAs may be involved in the development of prostate cancer,and they may be important biomarkers for diagnosis and prognostic assessment of prostate cancer.