| 空肠弯曲菌pcDNA3.1(-)-peb1A诱导的T细胞活化增殖研究 |
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| 引用本文： | 覃明,王宜峰,孙万邦,杜联峰,徐岗村,罗军敏,姚新生. 空肠弯曲菌pcDNA3.1(-)-peb1A诱导的T细胞活化增殖研究[J]. 免疫学杂志, 2011, 0(10): 857-860,865 |
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| 作者姓名： | 覃明 王宜峰 孙万邦 杜联峰 徐岗村 罗军敏 姚新生 |
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| 作者单位： | 遵义医学院免疫学教研室贵州省免疫学研究生教育创新基地; |
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| 基金项目： | 贵州省优秀人才省长基金项目(黔科教办2006[07]) |
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| 摘 要： | 目的探讨空肠弯曲菌(Campylobacter jejuni,CJ)pcDNA3.1(-)-peb1A疫苗免疫昆明种小鼠后T细胞活化增殖状态。方法重组质粒CJ pcDNA3.1(-)-peb1A免疫昆明种小鼠后,取其脾淋巴细胞,应用ELISA法检测其膜表面分子CD3、CD4、CD8、CD25和CD28。结果检测结果显示:CD3检测,佐剂DNA组高于裸DNA组,在第10天有显著性差异(P<0.05);CD8检测,佐剂DNA组高于裸DNA组,在第10、20天均有显著性差异(P<0.05),裸DNA组也高于两对照组,但仅在第20天有显著性差异(P<0.05);CD25检测,裸DNA组高于NS组,但仅在第10天有显著性差异(P<0.05),佐剂DNA组高于裸DNA组,也仅在第20天有显著性差异(P<0.05);CD4及CD28检测,佐剂DNA组高于裸DNA组,在第10、20天均有显著性差异(P<0.05);在第10、20天各脾淋巴细胞膜表面分子检测,佐剂DNA组高于两对照组,有显著性差异(P<0.05)。结论 CJ pcDNA3.1(-)-peb1A疫苗能够有效地诱导T细胞活化增殖。
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| 关 键 词： | 空肠弯曲菌 疫苗 peb1A |
Activation and proliferationof T lymphocyte induced by the DNA vaccine pcDNA3.1(-)-peb1A against CJ |
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| QIN Ming,WANG Yifeng,SUN Wanbang,DU Lianfeng,XU Gangcun,LUO Junmin,YAO Xinsheng Innovation Base of Immunology for Graduate,Education in Guizhou Province. Activation and proliferationof T lymphocyte induced by the DNA vaccine pcDNA3.1(-)-peb1A against CJ[J]. Immunological Journal, 2011, 0(10): 857-860,865 |
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| Authors: | QIN Ming WANG Yifeng SUN Wanbang DU Lianfeng XU Gangcun LUO Junmin YAO Xinsheng Innovation Base of Immunology for Graduate Education in Guizhou Province |
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| Affiliation: | QIN Ming,WANG Yifeng,SUN Wanbang,DU Lianfeng,XU Gangcun,LUO Junmin,YAO Xinsheng Innovation Base of Immunology for Graduate,Education in Guizhou Province,Department of Immunology,Zunyi Medical College,Zunyi 563003,China |
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| Abstract: | We aimed to explore activation and proliferation of T lymphocyte induced by the DNA vaccine pcDNA3.1(-)-peb1A against CJ on the Kunming mouse.Lymphocytes was gathered from spleens of the Kunming mice immunized with pcDNA3.1(-)-peb1A and then we assayed their surface molecules CD3,CD4,CD8,CD25 and CD28 with the method of enzyme-linked immunosorbent assay(ELISA).The results showed that:on the assay of CD3,the adjuvant DNA group was higher than the bare DNA group and it had a significant difference on the 10th... |
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| Keywords: | Campylobacter jejuni Vaccine peb1A |
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