细胞周期调控因子与喉癌癌变的相关性研究

目的 :探讨喉癌癌变过程中 Cyclin E、p2 1WAF1 /CIP1和 p5 3表达的临床病理学意义。方法 :用免疫组化检测 2 0例喉正常粘膜、40例喉不典型增生病变和 6 0例喉癌组织中 Cyclin E、p2 1WAF 1 /CIP1和 p5 3的表达。结果 :1Cyclin E阳性表达率在正常组、不典型增生组和喉癌组分别为 5 .0 % (1/ 2 0 )、2 0 .0 % (8/ 40 )、45 .0 % (2 7/ 6 0 ) ,喉癌组与正常组比较 ,其差异有极显著性意义 (P<0 .0 1) ;p2 1WAF1 /CIP1阳性表达率分别为 95 .0 % (19/ 2 0 )、75 .0 % (30 /40 )和 6 3.3% (38/ 6 0 ) ,喉癌组与正常组比较 ,其差异亦有显著性意义 (P<0 .0 5 ) ;p5 3阳性表达率分别为 0 (0 /2 0 )、30 .0 % (12 / 40 )和 6 1.7% (37/ 6 0 ) ,喉癌组与正常组比较 ,其差异有极显著性意义 (P <0 .0 1)。 2 p2 1WAF 1 /CIP1在高、中、低分化的喉癌中阳性表达率逐渐降低 ,其差异有显著性意义 (P <0 .0 5 )。 3 Cyclin E和 p5 3阳性表达显著相关 ,p2 1WAF 1 /CIP1与 p5 3阳性表达无相关性。结论 :1Cyclin E和 p5 3异常表达是喉癌发生中早期分子事件。 2p2 1WAF 1 /CIP1 表达与喉癌细胞分化程度有关。 3细胞周期因子相互调控机制在喉癌发展中起重要作用。

Study on the relation between the cell cycle regulators and laryngeal carcinogenesis

OBJECTIVE: To investigate the clinicopathological significance of the Cyclin E, p21WAF1/CIP1 and p53 expression in laryngeal carcinogenesis. METHOD: The expression of Cyclin E, p21WAF1/CIP1 and p53 was examined by using immunohistochemical method in normal epithelia (20), dysplastic epithelia (40) and laryngeal cancers (60). RESULT: 1. In normal epithelium, dysplastic squamous epithelium and in squamous cell carcinoma of the larynx, the positive immunostaining rate of Cyclin E protein was 5.0%(1/20), 20.0%(8/40) and 45.0%(27/60) respectively (P < 0.001), and p21WAF1/CIP1 protein was 95.0%(19/20), 75.0%(30/40) and 63.3%(38/60) respectively (P < 0.01), while the rate of p53 was 0, 30.0%(12/40) and 61.7%(37/60)(P < 0.001) respectively. 2. p21WAF1/CIP1 positive expression in highly, moderately and poorly differentiated carcinomas were 76.2%(16/21), 66.5%(19/29) and 30.0%(3/10) respectively (P < 0.05), p21WAF1/CIP1 protein expression was significantly associated with tumour cell differentiation. 3. The positive expression of Cyclin E closely related with the positive expression of p53 (P < 0.05). No correlation was found between p21WAF1/CIP1 expression and p53 expression. CONCLUSION: 1. Cyclin E and p53 could be an early molecular event in the carcinogenesis of larynx. 2. p21WAF1/CIP1 expression is associated with tumour cell differentiation. 3. The mutual mechanism of cell cycle regulators (Cyclin E, p21WAF1/CIP1 and p53) play an important role in the laryngeal carcinoma.