| 蒲公英根多糖的抗氧化活性研究 |
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| 引用本文： | 葛明明,缪月英,孙丽娜,李国清,胡北,高金波. 蒲公英根多糖的抗氧化活性研究[J]. 佳木斯医学院学报, 2014, 0(2): 39-41 |
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| 作者姓名： | 葛明明 缪月英 孙丽娜 李国清 胡北 高金波 |
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| 作者单位： | [1]黑龙江省教育厅生物药制剂重点实验室,佳木斯大学药学院,黑龙江佳木斯154007 [2]黑河学院物理化学系,黑龙江黑河164300 [3]哈尔滨学院,黑龙江哈尔滨150017 |
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| 基金项目： | 黑龙江省卫生厅项目,编号：2012-258; 佳木斯大学研究生创新课题,编号：YJSCX2012-032JD |
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| 摘 要： | 目的：提取蒲公英根中多糖,测定其多糖含量和体外抗氧化作用。方法：超声波协同酶法提取蒲公英根中的多糖,苯酚-硫酸法测定多糖的含量,以维生素C为对照,采用分光光度法测定蒲公英根多糖对OH·和O-2·自由基的抑制作用,HPLC法检测DPPH·的浓度法测定蒲公英根活性多糖清除DPPH·自由基的能力。结果：蒲公英根中的多糖含量为83.31%,清除OH·、O-2·和DPPH·的IC50值分别为：0.047mg/mL,0.01mg/mL,0.154mg/mL。结论：蒲公英根多糖对OH·、O-2·和DPPH·自由基均有良好的清除能力。
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| 关 键 词： | 蒲公英根 多糖 抗氧化活性 羟基自由基（·OH） 超氧阴离子自由基（·O2-） DPPH·自由基 |
Studies on antioxidant activity of polysaccharides from dandelion root |
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| Affiliation: | GE Ming - ruing,M IAO Yue - ying ,SUN Li - na ,LI Guo - qing ,HU Bei ,GAO Jin - bo (1. Key Laboratory of Biological Drugs, Education Departmenrt of HeiLongJiang province, Pharmacy College of Jiamusi University, Jiamusi 154007, China ! 2. Heihe University, Physical Chemistry Department, Heihe 164300, China 3. College of Harbin, Harbin 150080, China) |
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| Abstract: | Objective： To extract polysaccharides from Taraxaeums, and determine its content and study the Antioxidant activity of polysaccharides. Methods： Polysaccharides were extracted by ultrasonic synergy en- zyme methods. The contents of polysaccharide from Dandelion Root were determined by the method of phenol --sulphate acid. The Inhibition effect of polysaceharides to OH.and 0~- ~ were evaluated by spectrophotometry through comparing with VC. The concentration method of DPPH was detected by High Performance Liquid Chromatography. This methos can also be used to determine the ability of scavenging against DPPH agenstac- tivity polysaccharides from Dandelion Root, Results： The polysaccharide content is 63.31% in Taraxacums and IC50 for against OH., O2- ; and DPPH. were 0. 047mg/mL, 0.01 mg/mL,0. 154mg/mL. Conclusion： The polysaccharides had strong scavenging activity to O2-, OH. and DPPH.. |
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| Keywords: | taraxacum polysaecharide antioxidant activity hydroxyl free radical superoxideanion free radical DPPH free radical |
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