冷暴露对小鼠体内NK细胞和LAK细胞活性的影响

目的　探讨寒冷因素对自然杀伤细胞 (NK细胞 )和淋巴因子激活的杀伤细胞(LAK细胞 )活性的影响。方法　采用冷暴露方法处理小鼠 ,用乳酸脱氢酶 (LDH)释放法检测NK、LAK细胞的杀伤活性 ,并对结果进行分析。结果　短时间的急性冷暴露 ,NK及LAK细胞的活性呈一过性降低 ,以后又逐渐恢复并呈增强趋势。但是 ,在较长时间的冷暴露下 ,小鼠NK、LAK细胞的活性又显著降低 ,与对照组比较有显著差异 (p <0 .0 1)。结论　NK和LAK细胞的活性随冷暴露的时间而呈波动性变化 ,最终使机体的免疫功能降低。

Effects of cold exposure on the activities of NK and LAK cell in mouse

Objective To investigate effects of cold exposure on the activities of natural killer cells(NK cells) and lymphokine-activated killer (LAK) cell in mouse.Methods Forty-eight mice (Kunming strain) were randomly divided into cold exposure group and control group.We placed the cold exposure groups into -15±2℃ circumstance 1h everyday.After 3 days,1 week and 2 weeks,respectively,the mice were fed in normal temperature.Using lactate dehydrogenase (LDH) releasing methods to measure the activities of NK and LAK cell.Results After short-term cold exposure ,the activities of NK and LAK cell decreased temporarily. Then they came to recovery and had a enhancing trend.Compared with control group, the activities of NK and LAK cell decreased obviously(p<0.01) in long-term cold exposure group.Conclusion The activities of NK and LAK cell fluctuates with the cold exposure time and finally,the functions of immune system is decrease.