乙型肝炎HBV—DNA与HBs特异性免疫复合物的测定及意义

目的：了解各型乙型肝炎（乙肝）中HBV－DNA的含量与HBs特异性免疫复合物（HBs-IC)的关系及其意义。方法：共检测172例血清标本。用定量PCR测HBV－DNA及ELISA法测HBs-IC。结果：（1）、各型乙肝患HBs-IC阳性率和含量都明显升高，但HBV－DNA阳性组的乙肝患升高更为显；HBs-IC与HBV－DNA的测定结果无明显相关；（2）、HBV－DNA阳性率在不同肝病有所不同；在HBV－DNA阳性组中，DNA复制量在各病型无明显差异；（3）、HBs-IC检出率在无症状携带最低（59％）；慢性乙肝轻度、中度和原发性肝癌检出率中等（60％、75％及80％）；慢性乙肝重度，乙肝后肝硬化及急性乙肝检出率最高，均为100％。结论：在免疫应答功能正常的机体中，HBV－DNA的扩增常伴有HBs-IC的升高。推测：HBs-IC产生主要与机体的免疫功能有关，对HBV－DNA的复制及清除有一定意义。

Detection and Significance of HBV-DNA and HBsAg Circulating Immune Complexes in Viral Hepatitis B

Objective:To study the relationship between HBV-DNA content and HBsAg circulating immune complexes (HBs-IC) in viral hepatitis B and its significance. Methods: ELISA and fluorescence quantitative PCR were used in the determination of HBs-IC and HBV-DNA respectively. Serum samples from 172 cases with various types of viral hepatitis B were analyzed.Results: 1.The positive rate and the level of HBs-IC were high in all hepatitis B patients,with more significant in HBV-DNA positive patients. There was no significant correlation between HBs-IC and HBV-DNA 2.The HBV-DNA positive rate was different in patients with various period of hepatitis B While in the HBV-DNA positive group,the HBV-DNA content showed no difference. 3.The HBs-IC positive rate was 59% in asymptomatic HBV carriers,60%,75% and 80% in patients with mild and moderate chronic hepatitis B and primary liver cancer respectively,and 100% in patients with severe chronic heputitis B,cirrhosis or acute hepatitis B. Conclusion:In individuals with normal immunity,the amplification of HBV-DNA is accompanied with the increase of HBs-IC. It suggests that HBs-IC plays a role in the duplication and elimination of HBV-DNA.