gastrokine1在胃癌组织中的表达及其与胃癌临床病理特征的关系

目的 探讨gastrokine 1在胃癌的发生、发展过程中的作用.方法 应用实时荧光定量逆转录聚合酶链反应(RT-PCR)和免疫组织化学方法检测52例诊断明确的胃癌患者的癌组织及癌旁组织中gastrokine 1的表达,并分析其与胃癌患者临床病理学特征的关系.结果 gastrokine 1基因和蛋白在胃癌组织中的表达均低于癌旁组织(基因△Ct值:9.07±5.06比0.85±3.79；蛋白表达阳性率:5.8％ (3/52)比86.5％ (45/52)],差异均有统计学意义(P＜0.01).胃癌组织中gastrokine 1基因的表达与肿瘤的位置、浸润深度、分化程度、淋巴结转移及分期,患者的性别和年龄以及术前外周血CEA及CA19-9水平均无关(P＞0.05),而幽门螺旋杆菌(Helicobacter pylori,HP)阳性胃癌患者的癌组织中gastrokine 1基因表达低于HP阴性者(△Ct值分别为11.14±5.19和7.67±4.18,P＜0.05).结论 gastrokine 1在胃癌组织中的表达显著降低,它作为一种保护性基因在胃癌的发生和发展中扮演着重要角色,它的保护作用可能因HP感染而减弱.

Expression of Gastrokine 1 in Gastric Cancer Tissues and Its Relationship with Clinicopathologic Characteristics

Objective To clarify the role of gastrokine 1 in the process of formation and development of gastric cancer.Methods The expressions of gastrokine 1 in gastric cancer and paracancerous tissues of 52 patients with gastric cancer were detected by real-time fluorescence quantitative polymerase chain reaction(RT-PCR) and immunohistochemistry.Meanwhile the relationship of the expression level of gastrokine 1 with clinicopathologic characteristics were analyzed.Results The expression levels of gastrokine 1 gene and protein in the gastric cancer tissues were significantly lower than those in the paracancerous tissues(P<0.01).No significant relationship was found between expression of gastrokine 1 gene and clinicopathologic features including tumor location,depth of invasion,differentiation,lymph node metastasis,tumor stage,gender,age,and preoperative peripheral blood CEA and CA19-9 levels(P>0.05, respectively).What’s more,the expression level of gastrokine 1 gene in gastric cancer tissues of Helicobacter pylori (HP)-positive patients was lower than that in the negative ones(P<0.05).Conclusions Gastrokine 1 may play a significant role as an anti-oncogene in the process of the formation and development of gastric cancer.Its effect may become weak due to HP infection in gastric cancer patients.