非发酵性豆制品中柠檬黄、胭脂红和日落黄的测定

目的:建立准确简便的用于非发酵性豆制品中柠檬黄、胭脂红和日落黄含量检测的高效液相色谱分析方法。方法:将样品粉碎均匀,加入乙醇+氨水+水(7+2+1)的提取液直接提取样品中的色素,采用反相高效液相色谱法,以甲醇-0.02 mol/L乙酸铵水溶液梯度洗脱,二极管阵列检测器检测。结果:柠檬黄、胭脂红、日落黄三种色素在浓度范围为0.1μg/ml～50μg/ml线性良好,样品加标回收率为84.1%～104.4%,方法相对标准偏差RSD<5%,方法最低定量限为柠檬黄0.15 mg/kg、胭脂红0.30 mg/kg、日落黄0.25 mg/kg。结论:该方法前处理简单、检测灵敏度高、重现性好。

Determination of lemon yellow,cochineal and sunset yellow in non-fermented bean products

Objective:To establish a high performance liquid chromatography method for determination of lemon yellow,cochineal and sunset yellow in non-fermented bean products.Methods: Chromatographic column of DIKMA C18(250 mm×4.6 mm,5 μm) was used at 30℃ and mobile phase was methanol-0.02 mol/L ammonium acetate at flow rate of 1.0 ml/min in the mode of linear gradient elution.The determination wavelengths were 430 nm,510 nm and 480 nm,respectively.Ethanol-ammonia water–water(7∶ 2∶ 1) was used as the extraction solvent.Results: The linearities of the method were good in range of 0.1 μg/ml～50 μg/ml with the correlation coefficients of 0.9999.The recoveries were 84.1%～104.4% and the relative standard deviations were less than 5%.The limits of quantification were 0.15 mg/kg for lemon yellow,0.30 mg/kg for cochineal and 0.25 mg/kg for sunset yellow,respectively.Conclusion: The method is simple,sensitive and repeatable and applicable for determination of lemon yellow,cochineal and sunset yellow in non-fermented bean products.