消郁安神胶囊的质量标准研究

目的:建立消郁安神胶囊的质量标准。方法:采用薄层色谱(TLC)法对方中酸枣仁、知母、远志、西洋参进行定性鉴别;用高效液相色谱法测定西洋参中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1含量。结果:TLC斑点清晰、分离度好,阴性对照无干扰;人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1进样量分别在0.31～9.30、0.27～8.10、0.23～6.90μg范围内与各自峰面积积分值呈良好线性关系(r分别为0.9998、0.9998、0.9997);平均加样回收率分别为99.83%、99.28%、98.96%,RSD分别为0.75%、0.98%、0.64%(n均为9)。结论:所建标准可用于消郁安神胶囊的质量控制。

Study on Quality Standard of Xiaoyu Anshen Capsules

OBJECTIVE:To establish the quality standard of Xiaoyu anshen capsules. METHODS:TLC method was used to qualitatively identify Ziziphus jujuba,Anemarrhena asphodeloides,Polygalae Radix and Panax quinque folium;and the contents of ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 in Panax quinque folium were determined by HPLC. RESULTS:TLC sports were fairy clear and well-separated without inference from negative control;the linear ranges were 0.31～9.30 μg(r=0.999 8) for ginsenoside Rg1,0.27～8.10 μg(r=0.999 8) for ginsenoside Re and 0.23～6.90 μg(r=0.999 7) for ginsenoside Rb1,respectively. Average recoveries were 99.83%(RSD=0.75%) ,99.28%(RSD=0.98%) and 98.96%(RSD=0.64%) ,respectively(n=9) . CONCLUSION:The established standard can be used for the quality control of Xiaoyu anshen capsules.