炭疽芽孢杆菌传统鉴定指征与毒力基因关系的评价

目的 重新评估炭疽芽孢杆菌传统鉴定指征对确定具有致病能力的炭疽芽孢杆菌的意义。方法 采用常规的细菌学方法和PCR扩增毒力基因检测，并根据暗否具有毒力基因。比较传统鉴别指征的表现差异。结果 菌落形态，溶血与动力阴性，是与毒力基因存在相关的最得要特征。结论 在判断炭疽芽孢杆菌对人与环境的危害时，首先应确定是否存在致病性决定基因，在无条件进行这种检测时，数种传统的鉴别方法具有一定的参考价值。

Evaluation of the relation between the traditional index on the identification of Bacillus anthracis and its virulence determinant genes

Objective To evaluate the significance of traditional index on the identification of Bacillus anthracis and its correlation with pathogenic strains. Methods Routine bacteriologic methods and PCR of virulence genes were used to determine the difference on traditional identification index between pathogenic and nonpathogenic Bacillus. Results The characteristics of colony, with hemolysis and mobtility both negative are the important interrelated characters of pathogenic strains of Bacillus anthracis . Conclusion To judge the risk of anthrax, virulence of genes must be first defined. Some traditional methods for identification are still useful when molecular biological methods are not available.