脂联素基因多态性与胰岛素抵抗和2型糖尿病关系的研究

目的：探讨脂联素基因（APM-1）单核苷酸多态性（SNP）＋276G／T在天津地区汉族人群中的分布情况及其与胰岛素抵抗和2型糖尿病的关系。方法：采用聚合酶链式反应-限制性片段长度多态性（PCR—RFLP）技术对196例2型糖尿病（T2DM）患者和165例糖耐量正常（NGT）人群APM—1基因SNP276进行分析，通过放免法测定血浆脂联素水平，并对各受试者相关临床指标进行检测。结果：（1）SNP276 3种基因型在T2DM组和NGT组间总体分布无统计学差异，但T2DM组中T等位基因频率显著低于NGT组（24．74％VS32．42％，P〈0．05）。（2）两组中，GG型BMI、FPG（仅见于T2DM组）、FINS、TG（仅见于NGT组）、HOMA—IR显著高于TT型，HDL-C、血浆脂联素水平显著低于TT型。（3）相关分析和多元逐步回归分析表明T2DM组中血浆脂联素水平与FPG和HOMA—IR呈显著负相关，HOMA—IR是影响脂联素水平最显著的因素。结论：APM—1基因SNP276与天津地区汉族人群2型糖尿病及胰岛素抵抗具有相关性。

The association of adiponcetin gene polymorphism with insulin resistance and type 2 diabetes mellitus

Objective： To investigate the distribution of single-nucleotide polymorphism （SNP）＋276G/T in adiponectin gene in people of Han nationality in Tianjin Area and its association with insulin resistance and type 2 diabetes mellitus（T2DM）. Methods： 196 patients with T2DM and 165 subjects with normal glucose tolerance （NGT） were genotyped for SNP276 of APM-1 by PCR-RFLP. Plasma adiponectin concentrations and a homeostasis assessment of insulin resistance（HOMA-IR） and related clinical metabolic param- eters were measured. Results： （1） There was no statistically significant differences in genotype distributions of SNP in the two groups. However, the T allele frequency was significantly decreased in T2DM group （24.74% vs. 32.42%, P〈0.05）. （2） In both NGT and T2DM groups, the plasma level of adiponection, BMI, FPG （only in T2DM group）, FINS, TG（only in NGT group）, HDL-C and HOMA-IR were significantly different in GG genotype carriers compared with tit genotype carriers. （3） Significant association was found between plasma adiponection concentration and FPG and HOMA-IR in T2DM group. Multiple stepwise regression analysis indicated that the HOMA-IR was the most important factor impact on plasma adiponection. Conclusion： SNP276 in adiponectin gene is associated with type 2 diabetes mellitus and insulin resistance.