药物抑制兔耳病理性瘢痕中结缔组织生长因子的实验

目的探讨能有效抑制结缔组织生长因子（CTGF）的药物，以便为治疗病理性瘢痕提供依据。方法选择24只大耳白兔建立兔耳病理性瘢痕模型，随机分为4组：A组注射CTGF反义寡核苷酸，B组注射复方倍他米松，C组注射醋酸曲安奈得，D组为对照组，仅注射生理盐水。通过原位杂交法分别检测瘢痕组织中不同治疗组不同时段的CTGF表达，并通过苏木精-伊红（HE）染色法检测瘢痕组织中不同治疗组不同时段的成纤维细胞数。结果A、B、C3组在同一时段其CTGF表达均比D组低，差异有统计学意义（P〈0．05），A组较B、C两组CTGF表达低，差异也具有统计学意义（P〈0．05）。B、C两组之间CTGF表达差异无统计学意义（P〉0．05）。成纤维细胞计数结果与以上结果基本一致。结论CTGF反义寡核苷酸、复方倍他米松、醋酸曲安奈得均可抑制病理性瘢痕中CTGF的表达，CTGF反义寡核苷酸抑制效果最明显。

Antisense CTGF inhibits the expression of CTGF in hypertrophic scar of rabbit ears

Objective To investigate a medicine which can inhibit the expression of connective tissue growth factor (CTGF) in hypertrophic scar of rabbit ears. Methods 24 bigears white rabbits were used to establish a model of hypertrophic scar of rabbit ears, which was randomly divided into four groups. The hypertrophic scar was injected intralesionally with antisense CTGF (group A), betamethason (group B), triamcinolone acetonide (group C) and physiological saline (group D). Some scar tissue samples were sectioned in every group when the scar was treated after 7, 14, 30, and 60 days, respectively. The expression of CTGF mRNA in the scar was assessed by in situ hybridization and hematoxylin and eosin stain (HE stain) in every samples. Results The expression of CTGF mRNA and the counting of fibroblasts decreased in group A, which showed statistical difference as compared with groups B, C and D. Conclusions The results suggesz that antisense CTGF is able to inhibit the proliferation process of hypertrophic scar in rabbit ears and to remarkably decrease the degree of fiborsis in the scar.