| 若参碱对K562细胞端粒酶hTERT—mRNA表达及其酶活性影响作用的研究 |
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| 引用本文： | 李旭芬 郑树 等. 若参碱对K562细胞端粒酶hTERT—mRNA表达及其酶活性影响作用的研究[J]. 癌症, 2001, 20(4): 391-393 |
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| 作者姓名： | 李旭芬 郑树 等 |
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| 作者单位： | [1]浙江大学医学院附属第二医院肿瘤，浙江杭州310009 [2]浙 |
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| 基金项目： | 浙江省自然科学基金项目（NO:395142） |
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| 摘 要： | 目的：观察苦参碱对K562细胞hTERT-mRNA表达及端粒酶活性的影响作用，并明确两者的相关性。方法：以0．1－2mg/ml苦参碱处理K562细胞48h后，RT－PCR检测其hTERT-mRNA表达，同时行TRAP－PCR－ELISA端粒酶活性检测。结果：K562细胞为一强端粒酶活必细胞株，在浓度分别为0．、0．5、1．0、2．0mg/ml苦参碱作用后，K562细胞hTERT-mRNA表达明显受抑，同时伴有端粒酶活性下降，抑制率分别为0．3％、13．0％、91．7％和98．6％。结论：苦参碱可降低K562细胞hTERT-mRNA表达，同时伴端粒酶活性下降；端粒酶活性强度与hTERT-mRNA表达有关。
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| 关 键 词： | 苦参碱 端粒酶 肿瘤细胞系 聚合酶链反应 hTERT－mRNA |
| 文章编号： | R73-36 |
| 修稿时间： | 2000-06-19 |
Effects of Matrine on hTERT- mRNA Expression and Telomerase Activity in K562 Cells |
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| LI Xu-fen. Effects of Matrine on hTERT- mRNA Expression and Telomerase Activity in K562 Cells[J]. Chinese journal of cancer, 2001, 20(4): 391-393 |
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| Authors: | LI Xu-fen |
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| Abstract: | Objective:This study was designed to investigate the effects of matrine on hTERT- mRNA expression and telomerase activity in K562 cells and their relationship. Methods: The hTERT- mRNA expression was determined by RT- PCR assay in untreated and matrine- treated K562 cells, and telomerase activity was analyzed by TRAP- PCR- ELISA assay. Results: The hTERT- mRNA expression of K562 cells was significantly inhibited when treatment with matrine, while telomerase activity was decreased. Conclusion: Matrine can inhibit the hTERT- mRNA expression and telomerase activity of K562 cells. Telomerase activity might be related to the expression of hTERT- mRNA. |
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| Keywords: | Matrine Telomerase Tumor Cell line Polymerase chain reaction |
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