食管癌C-erbB-2、p53表达及幽门螺杆菌L型感染的意义

目的 :探讨幽门螺杆菌L型 (Hp L)感染与食管癌的关系以及C erbB 2、p53表达在食管癌发生机制中的作用。方法 :应用免疫组化和革兰氏染色技术检测 1 2 6例食管癌和 40例对照组的Hp L型、C erbB 2、和p53基因蛋白 ,对Hp L型阳性和阴性组织的C erbB 2、和p53表达行比较分析。结果 :癌组的革兰氏染色L型检出率 (67.5 % )与对照组 (2 7.5 % )有显著性差异 (P <0 .0 5) ;与免疫组化Hp L型抗原表达阳性率 (65 .1 % )无显著性差异 (P >0 .0 5) ,Hp L型检出阳性率为 61 .9% (78/ 1 2 6)。癌组的C erbB 2、p53表达阳性率显著高于对照组。癌组中Hp L型感染阳性组的C erbB 2、p53表达阳性率也高于其Hp L型阴性组 ,其差异均有显著性 (P <0 .0 5)。表明Hp L型感染与食管癌相关 ,与食管癌的C erbB 2、p53过表达也相关。结论 :Hp L型与食管癌的发生有关 ,有可能通过致基因突变而涉及食管癌的发生过程

Detection and Significance of the Helicobacter Pylori L-form C-erbB-2 and p53 in Esophageal Cancer

Purpose: To investigate the correlation between helicobacter pylori L-form(Hp-L) infection in human esophageal carcinoma(EC) and the impact of Hp-L and C-erbB-2 and p53 on the carcinogenesis of EC. Methods: Gram stain and immunohistochermical techniques were to detect the Hp-L infection and the expression of C-erbB-2 and p53 protein oncoproteins in 126 tissues of esophageal squamous cell carcinoma and 40 normal tissues of esophageal squamous epithelials. Results: The prositive rate of the infection of bacterial L-form(67.5%) in cancer group was significantly higher than that in normal group(27.5%) with gram stain(P<0.05).The antigen of Hp-L in 65.1% cases was detected by immunohistochemical techniques.There was no difference between the results obtained from each staining method respectively(P<0.05).The positive rate of the infection of Hp-L was 61.9%(78/126) in cancer group.The positive rates of C-erbB-2,p53 were significantly higher in cancer group than that in normal group(P<0.05).In cancer group,the expression of C-erbB-2,p53 were significantly higher in Hp-L infection positive group than that in negative group(P< 0.05).It showed that Hp-L infection was correlated with EC and the expression of C-erbB-2 and p53 in EC.The increased epithelial cell proliferation may be influenced by Hp-L infection and was associated with an increased risk of EC. Conclusion: The pathogenetic mechanism may be that Hp-L infection promotes mutation of C-erbB-2 gene and p53 gene.