RP-HPLC法同时测定四季三黄片中5种指标成分

目的 建立RP-HPLC法同时测定四季三黄片(大黄、黄芩、栀子、黄柏)中栀子苷、盐酸小檗碱、黄芩苷、大黄素和大黄酚的方法.方法 采用Thermo-C18色谱柱(250 mm×4.6 mm,5.0 μm)；流动相为甲醇-0.1％磷酸水溶液,采用梯度洗脱,检测波长分别为240 nm、345 nm、278 nm、254 nm；体积流量1.0 mL/min.结果 栀子苷、盐酸小檗碱、黄芩苷、大黄素和大黄酚的线性范围分别为:6～60 μg/mL、5.4～54μg/mL、5.3～53 μg/mL、4.3～43 μg/mL、5.8～58 μg/mL (0.999 7 ＜r ＜0.999 9),平均回收率在98.0％和102％之间.结论 该法检测效率高,专属性强,重复性好,可用于四季三黄片中栀子苷、盐酸小檗碱、黄芩苷、大黄素和大黄酚的定量测定.

Determination of five components in Siji Sanhuang Tablets by RP-HPLC

AIM To establish a method for simultaneously determining geniposide,berberine hydrochloride,baicalin,emodin and chrysophanol in Siji Sanhuang Tablets(Rhei Radix et Rhizoma,Scutellariae Radix,Gardeniae Fructus,Phellodendri Chinensis Cortex).METHODS The separation was performed on a thermo-C18 column(250 mm×4.6 mm,5.0 μm) with gradient elution using methanol and water(contained 0.1% H3PO4)as mobile phase.The detection wavelengths were 240 nm,345 nm,278 nm and 254 nm,respectively.RESULTS Linear ranges of geniposide,berberine hydrochloride,baicalin,emodin and chrysophanol concentrations were 6-60 μg/mL,5.4-54 μg/mL,5.3-53 μg/mL,4.3-43 μg/mL,5.8-58 μg/mL(0.999 7