肠胃清粗提物协同奥沙利铂抑制人结肠癌HCT116细胞及其作用机制研究

目的 探讨中药复方肠胃清(黄芪、党参、白术、八月札、薏苡仁、野葡萄藤、大血藤、猪苓)粗提物与奥沙利铂联合应用抑制人结肠癌HCTll6细胞的协同增效作用及机理.方法 奥沙利铂、肠胃清提取物分别单独以及联合作用于人结肠癌HCTll6细胞,采用MTT法检测半数抑制质量浓度(IC50),通过TransweⅡ实验检测侵袭转移抑制作用,并利用Western Blotting技术检测侵袭转移与凋亡相关蛋白的表达变化.结果 肠胃清提取物、奥沙利铂以及联合肠胃清提取物(0.15 mg/mL)时奥沙利铂对人结肠癌HCTll6细胞的半数抑制质量浓度分别为(3.24±0.06) mg/mL、(15.74±0.53)μg/mL及(10.32±0.37)μg/mL,联合用药组半数抑制质量浓度降低,侵袭抑制率提高,ADAM-17、MMP-2、MMP-9、BCL-2蛋白表达下调(P＜0.05),P53蛋白表达升高(P＜0.01).结论 中药复方肠胃清粗提物具有一定的抗人结肠癌细胞HCTll6作用,在与奥沙利铂联用时表现出明显的协同作用,其机制可能与降低ADAM-17、M MP-2、MMP-9、BCL-2蛋白以及提高P53蛋白的表达有关.

Synergetic effect of Changweiqing Extract combined with oxaliplatin on human colon cancer HCT116 cells

AIM To explore the synergy and its mechanism of aqueous extract of Compound Changweiqing(Astragali Radix,Codonopsis Radix,Atractylodis macrocephalae Rhizoma,Akebiae Fructus,Coicis Semen,Vitis heyneanae Caulis,Sargentodoxae Caulis,Polyporus) combined with oxaliplatin against human colon cancer HCT116 cells.METHODS Human colon cancer HCT 116 cells were treated with Changweiqing Extract along or combined with oxaliplatin.MTT assay was used to detect half inhibitory concentration(IC50),Transwell assay for invasion and metastasis Western Blotting for the expression of invasion and metastasis-associated proteins and apoptosis-related proteins.RESULTS Half inhibitory concentrations of Changweiqing Extract,oxaliplatin and in combination with Changweiqing Extract(0.15 mg/mL) were(3.24±0.06) mg/mL,(15.74±0.53) μg/mL and(10.32±0.37) μg/mL,respectively,and half inhibitory concentrations of the combined treatment group were significantly low(F= 836.533,P <0.01),and invasion inhibition rate increased(F= 128.945,P <0.01).ADAM-17,MMP-2,MMP-9,BCL-2 protein expression in combined group were significantly lower compared with those in the other groups(F= 98.163,5.844,17.633,27.944,P <0.05),and P53 protein expression was significantly higher than that in the other groups(F=18.490,P <0.01).CONCLUSION Changweiqing Extract plays a clear role in treating human colon cancer HCT116 cells,which shows markedly synergistic effect when combined with oxaliplatin.The mechanism is due to reduction in the ADAM-17,MMP-2,MMP-9,BCL-2 protein expression and increases in P53 protein expression.