平消胶囊对胆管癌细胞生长及CD44v6蛋白表达的影响

目的通过观察平消胶囊(郁金、马钱子粉、仙鹤草、五灵脂、白矾、硝石、干漆、枳壳)血清作用于人肝外胆管癌细胞QBC939后,对癌细胞的生长和转移的影响,探讨其抗癌及转移机制。方法将平消胶囊灌喂大鼠,制备含药血清,血清培养胆管癌细胞,MTT法检测不同剂量含药血清对人胆管癌细胞QBC939的生长抑制作用,流式细胞仪检测细胞周期、细胞膜上黏附分子CD44v6表达的变化。结果平消胶囊血清能明显抑制人肝外胆管癌细胞QBC939的增殖,具有时间、剂量依赖关系。药物作用后,QBC939细胞生长受阻于G0/G1期,并且黏附分子CD44v6在细胞膜上表达下调,剂量越大,CD44v6下调越明显。结论平消胶囊能有效地抑制QBC939细胞增殖,通过影响细胞生长、诱导细胞膜上黏附分子CD44v6表达下调,导致细胞凋亡,防止癌细胞转移。

Effect of Pingxiao Capsule on cell growth and CD44v6 expression of cholangiocarcinoma cells

AIM To evaluate the growth-inhibiting and transfer-restraining actions of Pingxiao Capsule(Curcumae Radix,Strychni Semen pulveratum,Agrimoniae Herba,Trogopterori Faeces,Alumen,Kalii Nitras,Toxicodendri Resina,Aurantii Fructus) on human cholangiocarcinoma cell line QBC939 cultured in vitro and its mechanism for anticancer.METHODS Rats were perfused with Pingxiao Capsule,and drug-contained serums were prepared.And then the serums were incubated with cholangiocarcinoma cells.MTT assay was used to evaluate the inhibiting effects of drug-contained serums in different concentrations on QBC939 cells.The changes of cell cycle and the expression of adhesive molecule CD44v6 on cell membrane were analyzed by flow cytometry.RESULTS Pingxiao Capsule drug-serum which had relationship between obvious dose and time effect,could evidently inhibit the proliferation of QBC939 cells.After treating with the drug-serum,the QBC939 cells stopped in the G0/G1 phase in dose-time relation.Furthermore,the expression density of CD44v6 on cell membrane decreased with increasing of concentrations of Xiaoping Capsule.CONCLUSION Xiaoping Capsule could evidently inhibit the proliferation of QBC939 cells by induction of the apoptosis,reduce the expression of adhesive molecule CD44v6 on cell membrane,and consequently prevent QBC939 cells from cancerometastasis.