薏苡仁油对人原位胰腺癌BxPC-3细胞生长及VEGF和bFGF表达的影响

目的研究薏苡仁油(康莱特注射液)体外对人原位胰腺癌BxPC-3细胞生长和血管生长因子的影响,探讨薏苡仁油抗肿瘤作用机制。方法薏苡仁油作用BxPC-3细胞后,经瑞氏染色观察细胞形态,Hoechst 33258荧光染色、DNA梯度电泳观察细胞凋亡;流式细胞仪检测细胞周期的变化;ELISA法检测血管内皮生长因子(VEGF)、成纤维细胞生长因子(bFGF)的变化。结果薏苡仁油(2 mg/mL,20μL/mL)作用于BxPC-3细胞后,瑞氏染色可见细胞出现典型的凋亡小体,Hoechst33258荧光染色可见BxPC-3细胞出现特征性凋亡变化,琼脂糖电泳观察到明显的细胞凋亡特征性DNA梯形条带;细胞周期阻滞在G0/G1期;细胞上清液中VEGF、bFGF的表达水平明显下调。结论薏苡仁油能影响BxPC-3细胞生长周期,导致细胞周期阻滞,下调VEGF和bFGF的表达水平,可能对抑制胰腺癌细胞的扩散产生一定作用。

Effects of Coicis Semen oil on growth of human in situ pancreatic cancer cell line BxPC-3 and expression of VEGF and bFGF

Objective To investigate the effect of Coicis Semen oil(Kanglaite Injection) on the proliferation,apoptosis,and vascular endothelial growth factor(VEGF) of human in situ pancreatic cancer cell line BxPC-3 in vitro,and to explore its mechanism.Methods BxPC-3 cells in vitro were treated with Coicis Semen oil(2 mg/mL,20 μL/mL),cell morphology was observed by Wright’s staining;Apoptosis was assayed by Hoechst 33258 fluorescence staining and DNA gredient agarose gel electrophoresis;Diversification of cell cycle was detected by flow cytomletry;Changes of VEGF and fibroblast growth factor(bFGF) were detected by ELISA.Results Wright’s staining showed characteristic apoptotic body in BxPC-3 cells;Hoechst 33258 fluorescence staining showed characteristic change of apoptosis;Obvious DNA-ladder strap was observed by agarose gel electrophoresis;Cell cycle was retarded on G0/G1 stages;The level of VEGF and bFGF expression in the cell liquid supernatant has been a distinct decline.Conclusion Coicis Semen oil could affect the cell growth cycle of BxPC-3 cells,conduce the retardance of cell cycle,and reduce the level of VEGF and bFGF expression,which may help for restraining the pervasion of pancreatic cancer cells to some extent.