| HPLC法测定乌参醒脑滴丸中人参皂苷Rg1、Re、Rb1和Rd |
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| 引用本文： | 范辉,顾泉琳,贝伟剑.HPLC法测定乌参醒脑滴丸中人参皂苷Rg1、Re、Rb1和Rd[J].中成药,2012,34(7):1280-1283. |
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| 作者姓名： | 范辉 顾泉琳 贝伟剑 |
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| 作者单位： | 广东药学院中医药研究院国家中医药管理局高脂血症调肝降脂重点研究室/脂代谢三级实验室广东省代谢性疾病中医药防治重点实验室;广东药学院药科学院 |
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| 基金项目： | 广东药学院2007年度人才引进基金资助项目(2007ZY103);广州市科技计划资助项目(11C32130738) |
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| 摘 要： | 目的建立乌参醒脑滴丸(人参、首乌,银杏叶提取物)中人参皂苷Rg1、Re、Rb1和Rd的HPLC测定方法。方法采用Diamonsil-C18色谱柱(150 mm×4.6 mm,5.0μm),柱温30℃;流动相为乙腈和水进行梯度洗脱,体积流量为1.0mL/min;检测波长为203 nm。结果人参皂苷Rg1、Re、Rb1和Rd分别在11.68～58.4μg/mL、15.30～153.0μg/mL、13.25～132.5μg/mL、7.65～76.5μg/mL质量浓度范围内与峰面积呈良好的线性关系,4种皂苷类成分的平均回收率为97.5%(RSD为1.56%)、100.3%(RSD为2.13%)、97.6%(RSD为1.98%)、98.6%(RSD为1.53%)。结论该法灵敏、简便、准确,可用于乌参醒脑滴丸的质量控制。
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| 关 键 词： | 乌参醒脑滴丸 人参皂苷Rg1 人参皂苷Re 人参皂苷Rb1 人参皂苷Rd 高效液相色谱 |
Determination of ginsenoside Rg1,ginsenoside Re1,ginsenoside Rb1 and ginsenoside Rd1 in Wushen Xingnao Dropping Pill by HPLC |
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| FAN Hui,GU Quan-lin,BEI Wei-jian.Determination of ginsenoside Rg1,ginsenoside Re1,ginsenoside Rb1 and ginsenoside Rd1 in Wushen Xingnao Dropping Pill by HPLC[J].Chinese Traditional Patent Medicine,2012,34(7):1280-1283. |
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| Authors: | FAN Hui GU Quan-lin BEI Wei-jian |
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| Institution: | 1(1.Key Unit of Modulating Liver to Treat Hyperlipidemia/Three Level 3 Laboratory of Lipid Metabolism,SATCM,Guangdong TCM Key Laboratory Against Metabolic Diseases Institute of Chinese Medicinal Sciences,Guangdong University of Pharmacy,Guangzhou 510006,China;2.College of Pharmacy,Guangdong University of Pharmacy,Guangzhou 510006,China) |
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| Abstract: | AIM To establish an HPLC method for determining the contents of ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1 and ginsenoside Rd in Wushen Xingnao Dropping Pill(Ginseng Radix et Rhizoma,Polygoni multiflori Radix,Gingko leaves extract).METHODS Chromatography was performed on a Diamonsil-C18(150 mm×4.6 mm,5.0 μm) at 30 ℃.The mobile phase was composed of acetonitrile and water in gradient mode with a flow rate of 1.0 mL/min.The detection wavelength was set at 203 nm.RESULTS The calibration curves of ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1 and ginsenoside Rd were linear in the ranges of 11.68-58.4 μg/mL,15.30-153.0 μg/mL,13.25-132.5 μg/mL,7.65-76.5 μg/mL,respectively.The average recoveries of ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1,ginsenoside Rd were 97.5%,100.3%,97.6%,98.6%,respectively.And RSDs were 1.56%(n=6),2.13%(n=6),1.98%(n=6),1.53%(n=6),respectively.CONCLUSION The method can be used for quantitative determination of the preparation. |
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| Keywords: | Wushen Xingnao Dripping Pill ginsenoside Rg1 ginsenoside Re ginsenoside Rb1 ginsenoside Rd HPLC |
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