黄精低聚糖的分离纯化及结构分析

目的 从黄精中分离纯化低聚糖样品并进行结构分析.方法 采用80％乙醇回流提取,D101大孔吸附树脂柱层析、活性炭柱层析和Sephadex LH-20凝胶柱层析分离纯化,得到黄精低聚糖样品.采用化学分析方法研究黄精低聚糖样品的理化性质,并通过高效凝胶渗透色谱(HPGPC)、气相色谱(GC)、紫外光谱(UV)、红外光谱(IR)、核磁共振氢谱(1HNMR)和核磁共振碳谱(13C NMR)等方法对黄精低聚糖样品进行结构分析.结果 黄精低聚糖样品纯度较高,为均一性组分,平均分子量为1 471Da,由单一葡萄糖组成的D-吡喃葡聚糖,分子结构中葡萄糖的连接方式主要为1→3和1→4,苷键构型以β-糖苷键为主,还含有少量的α-糖苷键.结论 首次从黄精中分离并鉴定了黄精低聚糖.

Isolation,purification and characterization of oligosaccharide from Polygonati Rhizoma

AIM To study the isolation,purification and characterization of Polygonati Rhizoma oligosaccharide(PRO).METHODS Polygonati Rhizoma was extracted by 80% ethanol,and further isolated and purified by D101 macroporous adsorption resin column chromatogramphy,activated carbon column chromatography and Sephadex LH-20 gel column chromatography.The structural analysis was identified by high performance gel permeation chromatography(HPGPC),gas chromatography(GC),ultraviolet spectrometry(UV),infared spectroscopy(IR),NMR spectroscopy(1H,13C).RESULTS PRO was a single homogeneous component and the average molecular weight was estimated at 1 471 Da.The D-pyran glucan consisted entirely of glucose units,which were linked in straight chains by 1→4 bond and 1→6 bond,most of the constituent glucosidic bond were jointed in β-form with a little of α-form.CONCLUSION The PRO is obtained from Polygonati Rhizoma for the first time.