白藜芦醇在大鼠心肌缺血再灌注损伤中的作用及机制

目的 研究白藜芦醇对大鼠心肌缺血再灌注损伤的保护作用,并进一步阐明其作用机制.方法 45只大鼠随机分为3组,假手术组、单纯缺血再灌注组、白藜芦醇预处理组,每组15只.结扎大鼠左冠状动脉前降支建立大鼠心肌缺血再灌注损伤模型.TUNEL法检测心肌细胞的凋亡,Western-blot检测Sirtl、P53及乙酰化P53的表达.结果 白藜芦醇能减少大鼠心肌缺血再灌注后心肌细胞的凋亡(P＜0.05),使Sirtl表达增高(P＜0.05),乙酰化P53表达降低(P＜0.05).结论 白藜芦醇对大鼠心肌缺血再灌注损伤具有保护作用,其机制可能与Sirtl-P53通路有关.

Effects of resveratrol on rat hearts impaired by ischemia/reperfusion

AIM To investigate the protective effects of resveratrol against myocardiac ischemia/reperfusion injury and its mechanism.METHODS Forty-five rats were randomly divided into three groups(15 rats each group): sham operation group,ischemia/reperfusion group,resveratrol pretreatment group.The myocardial ischemia-reperfusion injury model in rats was established by ligating left anterior descending coronary artery.TUNEL method was used to detect myocardial cell apoptosis.Protein expression of Sirt1,P53 and acetylation P53 was measured by Western blot.RESULTS The experiment revealed that resveratrol inhibited myocardial cell apoptosis(P<0.05),increased Sirt1 level(P<0.05),and decreased the acetylation P53 content(P<0.05) on rat hearts impaired by ischemia/reperfusion.CONCLUSION Resveratrol shows protective effects against myocytes ischemia/reperfusion injury induced by coronary ligation in rats,and its mechanism is probably related to Sirt1-P53 pathway.