青钱柳提取物对HepG2 2.2.15细胞HBsAg和HBeAg表达的影响

目的研究青钱柳提取物体外抗乙肝病毒作用。方法青钱柳用75%乙醇提取后按溶剂极性萃取,获得的三氯甲烷部位经柱层析得到Fr.2组分。体外培养HepG2 2.2.15细胞,MTT法检测青钱柳提取物Fr.2组分对HepG22.2.15细胞的细胞毒作用,用ELISA法检测HepG2 2.2.15细胞培养液中HBsAg和HBeAg的表达。结果青钱柳提取物Fr.2组分对HepG2 2.2.15细胞无明显的细胞毒作用。与对照组比较,Fr.2组分对HBsAg和HBeAg的表达均有显著性抑制作用(P<0.01或0.05)。结论青钱柳提取物体外具有较强的抗乙肝病毒作用。

Inhibiting effect of Cyclocarya paliurus extracts on HBsAg and HBeAg expression of the HepG2 2.2.15 cells

AIM To explore the anti-hepatitis B virus(HBV) effects of the Cyclocarya paliurus extract by using the HepG2 2.2.15 cell lines.METHODS The Cyclocarya paliurus was macerated with 75% ethanol and fractionated through solvent-solvent partitioning.The Fr.2 fraction was obtained from chloroform extract by column chromatography.HepG2 2.2.15 cells were cultured for in vitro anti-hepatitis B virus test.MTT chromatometry was used to analyse the cytotoxicity of Fr.2 fraction to HepG2 2.2.15 cell.ELISA was used to detect HBsAg and HBeAg expression in the HepG2 2.2.15 cell lines.RESULTS The Fr.2 fraction had obvious inhibitory effects on the expressions of HBsAg and HBeAg in HepG2 2.2.15 cell lines without cytotoxicity,as compared with those of the control group(P <0.01 or 0.05).CONCLUSION The Cyclocarya paliurus extracts have strong anti-HBV action in vitro.