生晒参乙醇提取物直接酶法转化及液质分析

目的比较糖苷酶制剂直接催化水解生晒参乙醇提取物中人参皂苷部位转化制备高活性次生皂苷的能力。方法利用7种糖苷酶制剂直接水解生晒参乙醇提取物中天然皂苷部位,建立HPLC法评价其转化能力及采用LC/MS联用分析对特征峰加以指认。结果建立的HPLC方法可同时测定8种皂苷成分。糖苷酶制剂对生晒参乙醇提取物中天然皂苷部位转化能力分别为蜗牛酶SE07,62.1%;果胶酶PE05,62.1%;果胶酶PE06,51.6%;白酒酶LE04,31.5%;纤维素酶CE02,22.6%;纤维素酶CE01,9.7%。结论糖苷酶制剂可以直接转化人参皂苷部位成高活性次生皂苷部位。蜗牛酶与果胶酶可以规模化制备次生皂苷。

Directly enzymatic conversion of alcohol extracts from sun-dried Panax ginseng and LC/MS analysis

AIM To compare the conversion of saponins in alcohol extracts from sun-dried Panax ginseng by the catalysis of seven glycosidase preparations to the secondary saponin on industrial scale.METHODS Seven glycosidase preparations were used to directly hydrolyze ginseng saponins in alcohol extracts from sun-dried Panax ginseng and to produce secondary saponins.HPLC analysis was developed to evaluate the conversion ability of glycosidase and LC/MS method was established to identify the chromatogram peaks.RESULTS HPLC could be used to simultaneously determine eight ginseng saponins in the extracts.The conversion ability of glycosidase to secondary saponins in the alcohol extracts was as follows: snail enzyme SE07 reached 62.1%,pectinase PE05 reached 62.1%,pectinase PE06 reached 51.6%,liquor enzyme LE04 reached 31.5%,cellulase CE02 reached 22.6% and cellulase CE01 reached 9.7%.CONCLUSION The glycosidases are able to directly transform the ginseng saponins into secondary saponins.Snail enzyme and pectinase can prepare secondary saponins in mass production.