淫羊藿苷元自微乳在CaCO2细胞模型的肠吸收特性初步研究

目的制备淫羊藿苷元自微乳,考察其理化性质及体外肠吸收特性。方法在考察淫羊藿苷元理化性质的基础上,以油酸乙酯为油相,聚山梨酯80为乳化剂,丙三醇为助乳化剂制备淫羊藿苷元自微乳,采用透射电镜及激光粒度分析仪测定其稀释后所得微乳的形态、粒径分布和Zeta电位,并采用Caco-2细胞模型初步分析其肠吸收特性。结果所制备的淫羊藿苷元自微乳稀释后,微乳平均粒径为55.6 nm,Zeta电位为30.8 mV,在Caco-2细胞模型上的表观渗透系数(Papp)为(3.52±0.3)×10 6cm/s。结论淫羊藿苷元自微乳制剂稳定,体外研究显示自微乳系统能够促进淫羊藿苷元在肠道的吸收。

Absorption characteristics in intestine of icaritin self-microemulsion via CaCO2 monolayer model

Objective To prepare icaritin self-microemulsion and investigate its properties and oral absorption characteristics in Caco-2 monolayer model.Methods To prepare icaritin self-microemulsion with ethyl oleate as oil phase,Polysorbate-80 as surfactant,and glycerol as cosurfactant.The appearance,particle diameter distribution,and Zeta potential of the diluted icaritin self-microemulsion were investigated by transmission electron microscopy and laser grain size analyzer,and the intestinal absorption was studied by using Caco-2 monolayer model.Results The mean particle diameter distribution of the diluted icaritin self-microemulsion was 55.6 nm,with Zeta potential of 30.8 mV.The apparent permeability coefficient(Papp) of the diluted microemulsion in Caco-2 monolayer model was(3.52 ± 0.3) × 10 6cm/s.Conclusion The icaritin self-microemulsion is stable and could significantly improve the absorption of icaritin in the intestinal tract.