岩黄连的抗乙肝病毒活性成分研究

目的研究岩黄连Corydalis saxicola生物碱中具有抗乙肝病毒活性的化学成分。方法利用柱色谱法分离纯化岩黄连总生物碱中的化学成分,根据化合物的理化性质与波谱数据进行结构鉴定;利用HepG 2.2.15细胞株对质量分数较高的化学成分进行抗乙肝病毒活性试验。结果从岩黄连总生物碱中分离鉴定了16个生物碱,分别为二氢血根碱(1)、d-紫堇碱(2)、旋卡文定碱(3)、stylopine(4)、6-丙酮基-5,6-二氢血根碱(5)、二氢白屈菜红碱(6)、四氢巴马汀(7)、adlumidine(8)、(-)-salutaridine(9)、巴马汀(10)、原阿片碱(11)、小檗碱(12)、coptisine(13)、thalifaurine(14)、dehydroapocavidine(15)和木兰花碱(16)。对质量分数较高的化合物5、6、8～11、13、16进行抗乙肝病毒活性试验,结果表明化合物5、8活性一般,化合物11、16活性较弱,化合物6对HBV具有较强的抑制作用,化合物9次之。结论化合物1、4～6、8、9、13、14和16均为首次从该药用植物分离得到,化合物10的质量分数较高,是该植物的主要成分,化合物6对HBV抑制活性较强,可能是该植物主要的活性成分。

Anti-hepatitis virus constituents from Corydalis saxicola

Objective To study the anti-HBV constituents from Corydalis saxicola.Methods The constituents were repeatedly purified by column chromatography and were identified on the basis of spectral analysis and comparison of their spectral data with those previously reported.Compounds isolated in large amounts were assayed against HBV.Results Sixteen compounds were identified to be dihydrosanguinarine(1),d-corydaline(2),cavidine(3),stylopine(4),6-acetonyl-5,6-dihydrosanguinarine(5),dihydrochelerythrine(6),tetrahydropalmatine(7),adlumidine(8),()-salutaridine(9),palmatine(10),protopine(11),berberine(12),coptisine(13),thalifaurine(14),dehydroapocavidine(15),and(+)-magnoflorine(16).Compounds 5,6,8—11,13,and 16 with high amounts were chosen to detect anti-HBV activities.Compounds 5 and 8 were moderately active,compounds 11 and 16 showed weak inhibitory effects,while compound 6 exihibited the most potent activity against HBsAg and HBeAg secretions in HepG 2.2.15 cell line,followed by compound 9.Conclusion Compounds 1,4—6,8,9,13,14,and 16 are isolated from C.saxicola for the first time.Compound 10 is the main constituent and compound 6 exihibits the most potent activity against HBV.