RASSF1A 基因在宫颈癌中的甲基化检测及临床意义

 目的 探讨宫颈癌组织中RASSF1A(Ras association domain family 1A)抑癌基因启动子甲基化状态及临床意义以及与高危型HPVs感染的关系. 方法 甲基化特异性聚合酶链反应(MSP)方法检测39例宫颈鳞癌及12例正常宫颈组织中RASSF1A基因甲基化状态.聚合酶链反应(PCR)方法检测宫颈癌组织中HPV16、18型的感染状况. 结果 39例宫颈癌组织中有11例可见异常甲基化(28.2%);12例正常宫颈组织均未见甲基化;宫颈癌组HPV感染率为69.2%;RASSF1A基因甲基化在淋巴结转移组(75.0%)高于淋巴结未转移组(9.1 %),P<0.05.RASSF1A甲基化在患者年龄、肿瘤大小、病理组织学分级、临床分期和HPVs感染组之间差异无统计学意义, P>0.05.结论 RASSF1A基因启动子区5′-CpG岛的高甲基化是导致RASSF1A基因失活的重要机制,可能参与宫颈癌的发生过程.

Detection of Promoter Hypermethylation of RASSF1A and Clinical Significance in Cervix Carcinoma

Objective 　To observe the methylation status of RASSF1A tumor suppressor gene promoter in human cervical carcinoma and its clinical significance and it s relationship with HPVs infection. Methods 　Methylation-specific PCR (MSP) assay was used to analyze the methylation status of promoter of RASSF1A gene in 39 cervical carcinoma and 12 normal cervical tissues. HPV-16 、18 type in cervical carcinoma was examined by polymerase chain reaction ( PCR) technique. Results 　Hypermethylation of RASSF1A was observed in 28. 2 %(11/ 39) of 39 cervical cancer cases. None of the normal cervical tissues was methylated. HPV-16 、18 type DNA was assessed in 69. 2 % of the cervical carcinoma. RASSF1A hypermethylation was also positively related with lymph node metastasis ( P < 0. 05) . However , there was no evidence to demonst rate the relationship between RASSF1A hypermethylation and the patient′s age , sizes of tumors , histological grade , stage and HPV infection ( P > 0. 05) . Conclusion 　Epigenetic change due to 5′-Cp G island methylation is the main cause of inactivation of RASSF1A gene which may be involved in cervical carcinoma pathogenesis and progress.