安宫牛黄丸对实验性大鼠脑缺血的保护作用

目的 研究安宫牛黄丸对实验性大鼠脑缺血的保护作用及机制.方法 将60只大鼠随机分为假手术组、模型组、安宫牛黄丸组、西药组、中西药组,采用线栓法建立大鼠局灶性脑缺血的动物模型,在脑缺血后6 h、24 h、48 h、72 h进行神经功能行为评分;造模连续给药4 d后,取脑组织,计算脑系数,干湿重法观察脑组织含水量,腹主动脉采血以酶联免疫吸附法（ELISA法）检测血清中白细胞介素-10（IL-10）水平的变化.结果 阻断大脑中动脉后4 d内,除了假手术组外,所有大鼠都出现程度不同的运动障碍;脑系数、脑含水量和血清IL-10水平均显著升高（P〈0.05）;安宫牛黄丸组、西药组、中西药组均可不同程度地降低脑系数、脑组织含水量及改善神经功能缺损症状,并升高血清IL-10水平（P〈0.05）;以中西药组作用最优,与假手术组、模型组、安宫牛黄丸组、西药组比较有统计学意义（P〈0.05）;安宫牛黄丸组与西药组间相比差异无统计学意义（P〉0.05）.结论 安宫牛黄丸对局灶性脑缺血大鼠脑损伤具有保护作用,其对血清细胞因子的影响是其对脑缺血保护作用的可能机制之一.

The Protective Effect of Angong Niuhuang Pill on Experimental Cerebral Ischemia

Objective To investigate the protective effect of Angong Niuhuang pill on experimental cerebral ischemia and its mechanism.Methods Rats were randomly divided into five groups：Sham group,model group,Angong Niuhuang pill group,western medicine group,Chinese traditional medicine with western medicine group.The models with focal cerebral ischemia of rat were established by the suture occluded method.Neurology evaluation was studied at 6 h,24 h,48 h and 72 h after middle cerebral artery occlusion（MCAO）and cerebral ischemia.The coefficient of brain and water content in whole brain were examined.The level of serum interleukin 10（IL-10） in abdominal aorta was detected by enzyme linked in ununosorbent assay.Results Within four days of MCAO,different degrees of motion disturbances were observed in all rats except sham group.The coefficient of brain,the water content of whole brain and the level of serum IL-10 were obviously increased（P0.05）.Angong Niuhuang pill,western medicine with traditional medicine,and western medicine could decrease the cerebral coefficient and cerebral water content,improve neurologic deficit and increase the serum IL-10（P0.05）.Traditional medicine with western medicine showed significant effect compared with other groups.There was no difference between Angong Niuhuang Pill and western medicine.Conclusion The Angong Niuhuang pill could protect brain cell in rats with cerebral focal ischemia.