银杏酮酯对离体心肌缺血再灌注损伤血流动力学的影响

目的 在离体大鼠心肌缺血再灌注模型上,观察银杏酮酯（GBE50）对大鼠离体心肌缺血再灌注损伤的血流动力学的影响,并探讨其机制.方法 建立改良的Langendorff离体大鼠心脏灌注模型,平衡灌注30 min,缺血30 min,再恢复常速灌注40 min,造成心肌缺血再灌注损伤.用3组不同浓度的银杏酮酯 （12.5 mg/L、25 mg/L、50 mg/L）进行灌流,观察停灌前、再灌5 min、再灌10 min、再灌20 min、再灌40 min时,心率（HR）、左心室收缩峰压平均值（mLVSP）、左心室等容收缩和舒张期压力平均值（±dp/dtmax）的变化;冠脉流量（CF）、冠脉流出液超氧化物歧化酶（SOD）、乳酸脱氢酶（LDH）的变化.结果 用银杏酮酯灌流缺血的心脏后,与模型组比较,mLVSP、±dp/dtmax分别增加;平均冠脉流量增加,冠脉流出液中LDH含量均明显减少（P〈0.05或P〈0.01）,SOD含量显著升高（P〈0.05或P〈0.01）.结论 银杏酮酯对离体的缺血再灌注损伤心脏有保护作用,其作用可能与抗氧化机制有关.

Hemodynamic Effects of GBE50 on Ischemia-reperfusion Injury of Isolated Heart in Rats

Objective To investigate the effect of Ginkgo biloba extract 50（GBE50）on ischemia-reperfusion（IR） injury in heart of rats.Methods Langendorff rats model were built,then isolated rat hearts were subjected to reperfusion for 30 min,ischemia for 30 min,and reperfusion for 40 min.GBE50（12.5 mg/L,25.0 mg/L,50.0 mg/L） was given in reperfusion period.The heart rate（HR）,mean left ventricular systolic pressure（mLVSP）,the maximum change rate of left ventricular pressure rise and fall（±dp/dt）,coronary flow（CF）,superoxide dismutase（SOD）,lactate dehydrogenase（LDH） in coronary sinus effusion were observed.Results In isolated rat heart models,the ±dp/dtmax of the heart was increased.Compared with control group of ischemia-reperfusion,activities of LDH in effluent samples of GBE50 group were lower than that in ischemia-reperfusion group（P0.05 or P0.01）,and activities of SOD in effluent samples of GBE50 group were higher than that in ischemia-reperfusion group（P0.05 or P0.01）.Conclusion GBE50 might have myocardial protective effects during ischemia-reperfusion period.