| DNMT1与UHRF1基因在胃癌细胞系中的表达特点及其与癌细胞DNA甲基化的关系 |
| |
| 引用本文： | 王雪娇,张开立,王宏宇,胡先福,李世杰,王 茜,李 宏,孙 媛.DNMT1与UHRF1基因在胃癌细胞系中的表达特点及其与癌细胞DNA甲基化的关系[J].大连医科大学学报,2010,32(1):50-53. |
| |
| 作者姓名： | 王雪娇 张开立 王宏宇 胡先福 李世杰 王 茜 李 宏 孙 媛 |
| |
| 作者单位： | 1. 大连医科大学,临床医学系2007级
2. 辽宁省癌症基因组重点室及大连医科大学,细胞生物学教研室,辽宁,大连,116044 |
| |
| 基金项目： | 国家自然科学基金(No.30700365) |
| |
| 摘 要： | 目的]探讨DNMT1和UHRF1在胃癌的形成和转化中的表达特点和可能的生物学作用。方法]提取CIMP型H（High）的胃癌细胞系HGC-27和CIMP型为L（10w）的胃癌细胞系MGC803，BGC823及AGS的核酸样品进行UHRF1和DNMT1表达的分析。结果]DNMT1在MGC823和HGC-27表达水平明显高于其他胃癌细胞系（P〈0．01）；UHRF1在HGC-27中有表达水平高于其他3个胃癌细胞系（P〈0．01）。结论]胃癌细胞DNA的甲基化不但和DNMT1有关而且和UHRF1也有着密切关系。
|
| 关 键 词： | 胃癌 UHRF1 DNA甲基化 CIMP型 DNMT1 |
DNMT1 and UHRF1 expression patterns in gastric cancer cells and their correlation with DNA methylation |
| |
| WANG Xue-jiao,ZHANG Kai-li,WANG Hong-yu,HU Xian-fu,LI Shi-jie,WANG Qian,LI Hong and SUN Yuan.DNMT1 and UHRF1 expression patterns in gastric cancer cells and their correlation with DNA methylation[J].Journal of Dalian Medical University,2010,32(1):50-53. |
| |
| Authors: | WANG Xue-jiao ZHANG Kai-li WANG Hong-yu HU Xian-fu LI Shi-jie WANG Qian LI Hong and SUN Yuan |
| |
| Institution: | 1.Faculty of Clinical Medicine;College of Basic Medical Sciences;2.Liaoning Laboratory of Cancer Genomics and Department of Cell Biology;Dalian Medical University;Dalian 116044;China |
| |
| Abstract: | Objective] To elucidate DNMT1 and UHRF1 expression patterns of gastric cancers and their potential roles in gastrocarcinogenesis.Methods] Samples of RNAs were isolated from established human gastric cancer cell lines such as MGC803,BGC823 and AGS with CIMP-low and HGC-27 with CIMP-high DNA methylation patterns,and UHRF1 and DNMT1 expression was analyzed by RT-PCR.Results] DNMT1 was up-regulated in MGC823 and HGC cells,while it was expressed in low level in MGC803 and HGC-27(P<0.01).UHRF1 expression level... |
| |
| Keywords: | gastric cancer UHRF1 DNA methylation CIMP type DNMT1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《大连医科大学学报》浏览原始摘要信息 |
| 点击此处可从《大连医科大学学报》下载免费的PDF全文 |
