大豆异黄酮对肥胖大鼠低度炎症水平的影响

目的：探讨大豆异黄酮对膳食诱导的肥胖大鼠低度炎症介质水平的影响，以初步阐明大豆异黄酮改善肥胖性胰岛素抵抗状态的可能机制。方法：选用高脂饲料诱导的雄性SD肥胖大鼠，随机分为模型对照组和3个不同剂量大豆异黄酮组，同时另设基础饲料对照组。各组给予相应受试物1月，禁食过夜后股动脉采血处死各大鼠，分离肾周及睾周白色脂肪。以酶法检测各组动物空腹血糖，放射免疫法检测空腹血胰岛素、白介素-6及肿瘤坏死因子，酶联免疫法检测血清C-反应蛋白、抵抗素与脂联素含量。结果：与模型对照组比较：150mg／kg与450mg／kg组能明显降低大鼠空腹体质量、内脏脂肪沉积、血清胰岛素、白介素-6、肿瘤坏死因子-α含量，提高脂联素水平；450mg／kg组能明显降低血清抵抗素的含量；不同剂量大豆异黄酮组的C-反应蛋白水平与模型组比较无明显差异。结论：大豆异黄酮可能是通过减少大鼠体内脂肪沉积、调整脂肪源的低度炎症介质而改善肥胖大鼠胰岛素抵抗状态。

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OBJECTIVE: To explore the effects of soy isoflavone (SIF) on low-grade inflammation in obese rats induced by high-fat diet, and to elucidate mechanisms of SIF in improving insulin sensitivity. METHODS: Obese rats were randomly divided into 4 groups: One model control group and 3 SIF groups that were given water solutions with SIF at 0 mg/(kg x d), 50 mg/(kg x d), 150 mg/(kg x d), and 450 mg/(kg x d), respectively. After one month, fasting glucose, fasting insulin, interleukin-6, tumor necrosis factor-alpha, C-reactive protein, resistin, and adiponectin in serum were detected by enzymic method, radioimmunoassay, and enzyme linked immunosorbent assay, respectively. RESULTS: In the 150 mg/(kg x d) group and 450 mg/(kg x d) group, fasting body-weights, viscera fatty deposition, and contents of insulin, interleukin-6, and tumor necrosis factor-alpha in serum were significantly lower; serum adiponectin levels were significantly higher; and serum resistin levels were significantly lower in the 450 mg/(kg d) group than those of the model control group. There was no difference in serum C-reactive protein levels among the 3 SIF groups. CONCLUSION: Soy isoflavone may improve the insulin sensitivity by decreasing viscera fatty deposition and adjusting low-grade inflammatory molecules derived from white adipose tissues.