补肾通督胶囊对胶原诱导关节炎大鼠Th1／Th2细胞的影响

目的：从外周淋巴细胞比例、基因和血清细胞因子的角度研究补肾通督胶囊对胶原诱导关节炎大鼠TH1／TH2细胞的影响。方法：将42只大鼠，分为正常对照组（C）、模型组（M）、补肾通督胶囊的低剂量组（BSL）、中剂量组（BSM）、高剂量组（BSH）和雷公藤组（TG）。第1天进行造模，从第13天开始灌胃18d，在第31天取材，进行流式细胞术、RT-PCR和ELISA检测Th1和Th2细胞、IL-4和IFN-Y的基因表达及血清水平。结果：补肾通督组的Th1的百分比随着剂量的提高有下降的趋势，而Th2的百分则呈现上升的趋势。随着补肾通督剂量的提高，IFN-7的基因表达和血清水平呈现下降的趋势，而IL-4的基因表达和血清水平则有上升的趋势。结论：补肾通督随着剂量的提高，不但降低外周血中Th1细胞的表达，同时提高Th2细胞的表达，对IL-4和IFN-7的基因表达和血清水平皆有抑制Th1细胞效应和提高Th2细胞效应的趋势，提示其缓解RA的机制与调控Th细胞的分化与平衡密切相关。

The Effect of Bushen Tongdu Capsule on Th1/Th2 cell of Collagen Induced Arthritis Rat

Objective：To investigate the effect of Bu Shen Tong Du capsule （BS） on peripheral Th1/Th2 ratio, IFN-Y and IL-4 gene expression and serum IFN-Y and IL-4 level in collagen induced arthritis rat. Method： 42 rats were divided into normal control group （C）, model group （M）, low dose group of Bushen Tongdu capsule （BSL）, medium dose group （BSM）, high dose group （]3SH） and Tripterygium wilfordii group （TG）. After modeling on day 1, gavaged from the day 13 to the 31 , then based on materials drawing on the day 31, gene expression and serum level of Thl and Th2 cells,IL-4 and IFN- Y were detected through flow cytometry, RT-PCR and ELISA examination. Results： The percentage of Thl de- clined while Th2 rised when the dose increased in BS group. With the dose increasing, the Gene expression and serum lev- els of IFN-Y showed a decreasing trend while IL-4 were on the rise. Conclusion：With the dose of Bu Shen Tong Du capsule increased ,it not only reduced the expression of Thl in peripheral blood ,but also enhanced the expression of Th2 cells, the trend of Th1 cells inhibition and Th2 cells enhance were occured in both gene expression and serum levels of IL-4 and IFN-Y. Therefore, the therapeutic mechanism of RA control may be closely related to the modulation of Th cell differentiation and balance.