首页 | 本学科首页   官方微博 | 高级检索  
     

幽门螺杆菌相关性消化性溃疡的细胞凋亡研究
引用本文:陈晓秋,邓长生,朱尤庆. 幽门螺杆菌相关性消化性溃疡的细胞凋亡研究[J]. 临床消化病杂志, 2001, 13(3): 102-105
作者姓名:陈晓秋  邓长生  朱尤庆
作者单位:武汉大学第二临床学院消化内科
摘    要:目的:探讨幽门螺杆菌(Helicobacter pylori,HP)诱导胃上皮细胞凋亡的机制及细胞凋亡在HP相关性消化性溃疡(PU)中的作用。方法:内镜下胃粘膜活检取材,应用TUNEL法检测胃上皮细胞凋亡,免疫组化法检测凋亡调控蛋白bak、bcl-2的表达。结果:HP阳性胃溃疡(GU)和十二指肠(DU)患者细胞凋亡指数(AI)明显高于HP阴性非溃疡性消化不良(NUD)患者(P<0.01)。根除HP后,AI在DU明显下降(P<0.01),在GU无明显改变(P>0.05)。HP阳性DU溃疡活动期AI明显高于愈合期和瘢痕期(P<0.05),而HP阳性和GU溃疡三期AI无差异(P>0.05)。bak蛋白表达在HP阳性PU明显高于HP阴性NUD患者(P<0.05)。根除HP后,DU患者bak蛋白表达明显降低(P<0.05)。GU患者虽有下降,但无统计学差异。bcl-2蛋白表达在HP阳性PU与HP阴性NUD之间无显著差异,HP根除前后亦无明显改变。结论:HP诱导胃上皮细胞凋亡在十二指肠溃疡发病中起重要的作用,HP诱导胃上皮细胞凋亡可能是通过bak路径实现的。

关 键 词:幽门螺杆菌 细胞凋亡 十二指肠溃疡 胃溃疡 病理
修稿时间:2000-11-09

Studies on Gastric Epithelial Cell Apoptosis in HP Associated Peptic Ulcer
Chen Xiao qiu,Deng Chang sheng,Zhu You qing. Studies on Gastric Epithelial Cell Apoptosis in HP Associated Peptic Ulcer[J]. Chinese Journal of Clinical Gastroenterology, 2001, 13(3): 102-105
Authors:Chen Xiao qiu  Deng Chang sheng  Zhu You qing
Affiliation:Chen Xiao qiu,Deng Chang sheng,Zhu You qing Department of Gastroenterology,The Second Clinical College,Wuhan University,Wuhan,430071
Abstract:Purpose: To investigate the mechanism from helicobacter pylori(HP) that induced gastric epithelial cell apoptosis and the role of apoptosis in the development of peptic ulcer in HP infection. Methods: The density and distribution of the apopotic cells and the expression of bak and bcl 2 proteins were determined using terminal deoxynucleotidyl transferase mediated dUTP nick end labelling(TUNEL) and immunohistochemical approach in situ from biopsy specimens obtained by endoscopic examination. Results: The apoptotic index of HP associated gastroduodenal ulcer patients was much higher than that of HP negative non ulcer dyspepsia(NUD) patients(P<0.01). After eradication of HP, the apoptotic index of duodenal ulcer patients was distinctly decreasing(P<0.01), while there was no obvious change in gastric ulcer patients(P>0.05). In duodenal ulcer patients infected with HP, the patients with an active ulcer exhibited a significantly higher level of apoptosis than those with ulcers at both the healing and scarring stages(P<0.05), which was not found in three stages of gastric ulcer. The variations of bak protein expression was similar to that of apoptotic index(P<0.05). The expression of bcl 2 protein had no significant change between HP positive gastroduodenal ulcer patients and HP negative NUD patients. After eradication of HP, the expression of bcl 2 protein was also no obvious change in both duodenal ulcer patients and gastric ulcer patients(P>0.05). Conclusion: Gastric epithelial cell apoptosis induced by HP plays a crucial role in the development of duodenal ulcer and its realizition is by a bak dependent pathway.
Keywords:Helicobacter pylori Apoptosis Duodenal ulcer Gastric ulcer bak bcl 2  
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号