| 葛花提取物通过激活PPARγ上调ABCA1的表达而抑制THP-1源性泡沫细胞形成 |
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| 引用本文： | 朱容蓉,陈梦娇,赵真旺,刘嘉怡,吴剑锋,王宇菲,张敏. 葛花提取物通过激活PPARγ上调ABCA1的表达而抑制THP-1源性泡沫细胞形成[J]. 中国动脉硬化杂志, 2024, 32(5): 395-401 |
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| 作者姓名： | 朱容蓉 陈梦娇 赵真旺 刘嘉怡 吴剑锋 王宇菲 张敏 |
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| 作者单位： | 南华大学心血管疾病研究所 动脉硬化学湖南省重点实验室 湖南省动脉硬化性疾病国际科技创新合作基地 南华大学生物信息与医学大数据教研室,湖南省衡阳市 421001;湖北文明学院医学部基础医学院,湖北省襄阳市 441053;湖南医药学院公共卫生与检验医学院,湖南省怀化市 418000;南华大学附属第二医院心血管内科,湖南省衡阳市 421001 |
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| 基金项目： | 湖南省教育厅科学研究项目(19B490)；湖北省自然科学基金青年项目(2023AFB043、2021JJ40485)；大学生创新创业项目(S202112214008) |
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| 摘 要： | 目的]探寻葛花提取物(PFE)对巨噬细胞源性泡沫细胞脂质蓄积的影响。 [方法]通过MTT法筛选PFE对THP-1源性泡沫细胞的作用浓度,采用油红O染色和胆固醇检测试剂盒检测细胞内脂质蓄积情况,采用胆固醇流出试剂盒检测细胞的胆固醇流出水平,使用RT-qPCR和Western blot检测mRNA和蛋白表达。 [结果]PFE显著降低THP-1源性泡沫细胞内的脂质蓄积。PFE不影响CD36、清道夫受体AⅠ(SR-AⅠ)、固醇调节元件结合蛋白2(SREBP2)及3-羟基-3-甲基戊二酰辅酶A还原酶(HMGCR)的mRNA表达,但能上调ATP结合盒转运体A1(ABCA1)的mRNA和蛋白表达水平(P＜0.05),促进巨噬细胞源性泡沫细胞胆固醇流出(P＜0.01)。PFE可以激活过氧化物酶体增殖物激活受体γ(PPARγ)的活性(P＜0.01)及上调其mRNA和蛋白表达水平(P＜0.05)。与PFE对照组相比,加入GW9662处理后PPARγ和ABCA1的蛋白表达水平均下降(P＜0.01),胆固醇流出水平降低(P＜0.01)。 [结论]PFE可显著改善THP-1源性泡沫细胞内的脂质蓄积,通过PPARγ上调ABCA1表达及其介导的胆固醇流出抑制泡沫细胞形成。
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| 关 键 词： | 葛花提取物 泡沫细胞 ATP结合盒转运体A1 过氧化物酶体增殖物激活受体γ |
| 收稿时间： | 2023-11-30 |
| 修稿时间： | 2024-02-08 |
Pueraria Lobata Flowers Extract inhibits THP-1-derived foam cell formation by activating PPARγ to upregulate the expression of ABCA1 |
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| ZHU Rongrong,CHEN Mengjiao,ZHAO Zhenwang,LIU Jiayi,WU Jianfeng,WANG Yufei,ZHANG Min. Pueraria Lobata Flowers Extract inhibits THP-1-derived foam cell formation by activating PPARγ to upregulate the expression of ABCA1[J]. Chinese Journal of Arteriosclerosis, 2024, 32(5): 395-401 |
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| Authors: | ZHU Rongrong CHEN Mengjiao ZHAO Zhenwang LIU Jiayi WU Jianfeng WANG Yufei ZHANG Min |
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| Affiliation: | Institute of Cardiovascular Disease, University of South China & Key Laboratory for Arteriosclerology of Hunan Province & Hunan International Scientific and Technological Cooperation Base of Arteriosclerotic Disease & Department of Bioinformatics and Medical Big Data, University of South China, Hengyang, Hunan 421001, China;Medical Department of Basic Medical College, Hubei University of Arts and Science, Xiangyang, Hubei 441053, China;School of Public Health & Laboratory Medicine, Hunan University of Medicine, Huaihua, Hunan 418000, China;Department of Cardiology, Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China |
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| Abstract: | Aim To explore the effect of Pueraria Lobata Flowers Extract (PFE) on lipid accumulation in macrophage-derived foam cells. Methods The concentration of PFE in THP-1-derived foam cells was screened by MTT, intracellular lipid accumulation was detected by oil red O staining and cholesterol detection kit, intracellular cholesterol efflux levels were detected by cholesterol efflux assay kit, RT-qPCR and Western blot were used to analyze mRNA and protein expression. Results PFE significantly reduced lipid accumulation in THP-1-derived foam cells. PFE did not affect the mRNA expression of CD36, scavenger receptor-AⅠ (SR-AⅠ), sterol regulatory element-binding protein 2 (SREBP2), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), but it could upregulate the mRNA and protein expression levels of ATP-binding cassette transporter A1 (ABCA1) (P＜0.05), and promote the intracellular cholesterol efflux of macrophage-derived foam cells (P＜0.01). PFE could activate the activity of peroxisome proliferator-activated receptor γ (PPARγ) (P＜0.01) and upregulate the mRNA and protein expression levels of PPARγ (P＜0.05). Compared with the PFE control group, the expression of PPARγ and ABCA1 proteins decreased and cholesterol efflux decreased after GW9662 treatment (all P＜0.01). Conclusion PFE could significantly prevent the lipid accumulation in THP-1-derived foam cells and inhibit the formation of foam cells by upregulating ABCA1 expression and cholesterol efflux mediated by PPARγ. |
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| Keywords: | Pueraria Lobata Flowers Extract foam cell ATP-binding cassette transporter A1 peroxisome proliferator-activated receptor γ |
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