食管鳞癌患者血清VEGF和一氧化氮含量测定及临床意义

背景与目的：肿瘤血管生成是肿瘤赖以生长和转移的基础,而血管内皮生长因子（vascular endothelial growth factor,VEGF)和一氧化氮（nitric oxide,NO)与血管新生密切相关,本实验旨在通过检测食管鳞癌患者血清中VEGF和NO的表达变化,分析其临床意义,为食管鳞癌病情判定及预后估计提供辅助手段。方法：分别采用酶联免疫吸附反应9enzyme-linked immunosorbant assay,ELISA)法和分光光度法检测50例食管鳞癌患者血清中VEGF和NO的含量,并分析其临床意义。结果：正常健康人血清中VEGF和NO含量与食管良性病变患者无显著差异（P＞0．05）;食管鳞癌患者血清VEGF和NO含量较健康人明显升高（P＜0．01）,且随着食管癌的分期、分级和淋巴结转移而显著增高（P＜0．01）,血清VEGF和NO含量呈正相关（P＜0．01）。结论：食管鳞癌患者血清中VEGF和NO表达的增高与肿瘤的发生、发展及恶性行为有关,可作为临床上判断预后的指标之一。

Serum levels of VEGF and nitric oxide in esophageal squamous carcinoma patients and the clinical significance

Background & Objective: Angiogenesis is necessary for the growth and metastasis of malignant tumor and is closely related to vascular endothelial growth factor (VEGF) and nitric oxide (NO). This investigation was aimed to detect the serum levels of VEGF and NO in squamous carcinoma patients and analyze the clinical significance for prognosis evaluation. Methods: Serum VEGF and NO levels were detected by enzyme linked immunosorbant assay (ELISA) and spectrophotometry, respectively, and their clinical significance was analyzed. Results: Serum levels of VEGF and NO in squamous carcinoma patients were significantly higher than that of in normal persons (P< 0.01). There was a positive correlation between serum VEGF, NO levels and tumor pathological phases, histological grades and lymph node metastasis (P< 0.01). There was also a positive correlation between serum VEGF level and serum NO level (P< 0.01). Conclusion: Serum levels of VEGF and NO in squamous carcinoma patients were significantly correlated to the growth, development and malignant behavior of tumor and thus may serve as the indexes for clinical prognosis evaluation.