CD4~+ CD25~+调节性T细胞在HIV/AIDS患者中的作用及其与HIV病毒载量的相关性研究

目的研究HIV感染者/AIDS患者外周血CD4~+CD25~+调节性T细胞(CD4~+CD25~+ regulatory T cell,Treg)频率、功能及其临床意义。方法选择31例HIV感染者/AIDS患者和30例健康对照者,采用流式细胞仪检测各组外周血Treg的表型和频率。采取MACS磁珠分选Treg,利用~3H]胸腺嘧啶掺入法检测Treg在特异性HIV抗原刺激下对CD4~+CD25~+T细胞的增殖影响。结果HIV/AIDS患者组与正常对照组相比较,外周血CD4~+CD25~+调节性T细胞在统计学上无显著性意义。与正常对照组比较,HIV感染者外周血Treg比例升高,差异有统计学意义(P<0.01);与正常对照组比较,AIDS患者外周血Treg比例降低,差异有统计学意义(P<0.0001)。HIV RNA病毒载量与患者外周血Treg细胞数量呈正相关性(P<0.01)。Treg具有抑制HIV特异性的CD4~+CD25~- T细胞的增殖作用。结论HIV感染者/AIDS患者的细胞免疫功能紊乱,Treg能抑制HIV感染者/AIDS患者的HIV特异性细胞免疫反应,促进HIV病毒复制,与形成持续HIV感染有关。

Study on the role for CD4~+CD25~+ regulatory T cell in HIV infection/AIDS and the correlation between CD4~+ CD25~+ regulatory T cell and HIV viral load

Obiective To study on the frequency,phenotype,function and clinical signifi- cance of CD4~+CD25~+ regulatory T cell(Treg) in peripheral blood from patients with HIV infection/ AIDS.Methods Selection of 3 lpatients with human immunodeficiency virus (HIV) infection/ acquired immunodeficiency syndrome (AIDS)and 30 normal subjects (NS),The frequency and phenotype of Treg in peripheral blood of the patients and normal subjects were detected by flow cytometry.Isolation of Treg by magnetic activated cell sorter(MACS) assay,and investigation of the effect for Treg on CD4~+CD25~-T cell proliferation after HIV antigen stimulation by 3H] thymidine. Results The proportion of CD4~+CD25~+T cell in peripheral blood from patients with HIV infection /AIDS had no significant difference compared with NS.The proportion of CD4~+CD25~+ T cell in peripheral blood from patients with HIV infection were significantly increased compared with NS (P<0.01).But the Treg in peripheral blood from patients with AIDS were significantly increased in contrast to NS(P<0.0001).These was a positive correlation between HBV DNA viral load and Treg frequency.Treg could specifically inhibit the proliferation of CD4~+CD25~+ T cell.Conclusions The cellular mediated immune function of patients with HIV infection/AIDS was disordered.Treg could specially inhibit cellular mediated immune response and aggravate HIV replication and form HIV persistent infection.