壮阳药效动物实验评价方法的改进

目的 对壮阳药效评价方法进行优化,以探求更为有效的候选药物筛选方法.方法 模型组大鼠均灌胃给予西地那非10 mg/kg,对照组大鼠给予相同容量的生理盐水灌胃,每天1次,连续灌胃给药14 d.每次给药后连续记录2h内大鼠阴茎勃起、阴部理毛、爬背次数和参与动物数,并计算PEI值.采用改良的壮阳药效评价方法,整个过程采用摄像头观察并在不同时间段给予雌鼠诱导.结果 在雌鼠诱导组中,模型组大鼠给药后2 h PEI值较对照组明显升高(P＜0.05).在模型组中,与人为观察组相比,摄像头观察组大鼠给药后2 h PEI值明显升高(P＜0.05)；与非雌鼠诱导组相比,雌鼠诱导组大鼠给药后2 h PEI值明显升高(P＜0.05)；与持续诱导组相比,间断诱导组2h PEI值无明显差异,但60 ～ 120 min时间段PEI值明显升高(P＜0.05).结论优化后的壮阳药效评价方法能更为有效的评价候选药物的壮阳药效.

Seek the More Effective Screening Method on the Assessment Methods of Strengthening Yang Efficacy

Objective Optimization on the assessment methods of strengthening yang efficacy to seek the more effective screening method.Methods Rats of model groups were given silaenafil 10mg/kg by gavage.Rats of control groups were given normal saline only.All rats were gavaged once a day for two weeks.Rats were observed for 2 hours after each drug administration for penile erection,genital grooming and possible copulation mounting.The number of responding rats was recorded along with the number of sexual activity episodes(penile erection,genital grooming or copulation mounting).Penile erection index(PEI) was calculated for each group by multiplying the percentage of active rats(responding rats) by the total number of activity episodes.By using the modified assessment method,male rats were observed by camera.Meamwhile,we also stimulated the male rats by female rats at different periods.Results Among female rat-stimulated groups,the model groups compared with the control groups,PEI was significantly increased(P<0.05).Among the model groups,the camera observed group compared with the human observed group,PEI was significantly increased(P<0.05).Compared with non-female rat-stimulated group,PEI was significantly increased in female rat-stimulated group(P<0.05).The 2 h PEI showns no significant differences between sustained induction group and intermittent group.But,during the period of the second hour,PEI was significantly increased in intermittent group(P<0.05).Conclusions After optimized,the assessment methods can evaluated the strengthening yang efficacy of candidate drugs more effective.