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| 肉桂酸对A549细胞分化及端粒酶活性的影响 | |
| 引用本文： | 张婷,周舫,陈晓岚,刘红霞,吴逸明,赵玉芬. 肉桂酸对A549细胞分化及端粒酶活性的影响[J]. 郑州大学学报(医学版), 2003, 38(6): 905-907 |
| 作者姓名： | 张婷 周舫 陈晓岚 刘红霞 吴逸明 赵玉芬 |
| 作者单位： | 1. 郑州大学化学系,郑州,450052 2. 郑州大学公共卫生学院劳动卫生学教研室,郑州,450052 3. 郑州大学化学系,郑州,450052;清华大学化学系生命有机磷教育部重点实验室,北京,100084 |
| 基金项目： | 河南省卫生科研基金资助项目 2 0 0 13 5 |
| 摘 要： | 目的：探讨肉桂酸对人肺腺癌A549细胞诱导分化及对端粒酶活性的影响。方法：应用1mmol／L，3mmol／L肉桂酸(CINN)分别处理A549细胞，并设空白对照，观察体外细胞增殖情况，细胞分裂指数，流式细胞仪测定细胞周期，以TRAP-银染技术的非放射性方法检测端粒酶活性。结果：CINN作用后的A549细胞，细胞增殖明显受到抑制；细胞分裂指数减少；细胞周期出现向G1／G0期移行的特征性动力学改变；相应的端粒酶活性下降，甚至活性丧失。结论：肉桂酸能诱导A549细胞的分化和降低端粒酶活性。 |
| 关 键 词： | 肉桂酸 肺肿瘤细胞 诱导分化 端粒酶 |
| 修稿时间： | 2003-03-25 |
Effects of cinnamic acid on telomerase activity and cell differentiation in A549 cells |
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| ZHANG Ting ),ZHOU Fang ),CHEN Xiaolan ),LIU Hongxia ),WU Yiming ),ZHAO Yufen ,) ). Effects of cinnamic acid on telomerase activity and cell differentiation in A549 cells[J]. Journal of Zhengzhou University: Med Sci, 2003, 38(6): 905-907 | |
| Authors: | ZHANG Ting ) ZHOU Fang ) CHEN Xiaolan ) LIU Hongxia ) WU Yiming ) ZHAO Yufen ) ) |
| Affiliation: | ZHANG Ting 1),ZHOU Fang 2),CHEN Xiaolan 1),LIU Hongxia 1),WU Yiming 2),ZHAO Yufen 1,3) 1)Department of Chemistry,Zhengzhou University,Zhengzhou 450052 2)Department of Labor-Health,College of Public Health,Zhenzhou University,Zhengzhou 450052 3)The Key Laboratory for Bioorganic Phosphorus Chemistry,Tsinghua University,Beijing 100084 |
| Abstract: | Aim:To study the differentiation effect of cinnamic acid (CINN) on human lung adenocarcinoma A549 cells and the possible mechanism. Methods: A549 cells were treated by CINN (1mmol/L, 3mmol/L), the cell growth rate was determined by cell counting, and its viability was determined by trypan-blue exclusion assay. Flow cytometry (FCM) was used to analyze the cell cycle. The telomerase activity was detected by the non-radioactive method (silver staining method) during the course of differentiation of A549 cell line.Results:After A549 cells were incubated with CINN, the cell proliferation was markedly inhibited; the cell division index of the CINN groups was lower than that of the control group; cell cycle showed G1/G0 phase stasis; the telomerase activity in different CINN groups changed markedly. Conclusion:CINN could inhibit the proliferation of A549 cells and the telomerase activity. |
| Keywords: | cinnamic acid lung neoplasm cell induced differentiation telomerase |
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